Preparation method of Jinchang jujube polysaccharide with immune modulating function and application thereof

A technology of immune regulation and jujube polysaccharides, which can be applied in drug combination, allergic diseases, etc., and can solve problems such as polysaccharide extraction research

Active Publication Date: 2018-11-27
TARIM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, in the prior art, the extraction of polysaccharides focuses on the purity and extraction rate of polysaccharides, and there is no research on the extraction of polysaccharides with specific biological functions

Method used

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  • Preparation method of Jinchang jujube polysaccharide with immune modulating function and application thereof
  • Preparation method of Jinchang jujube polysaccharide with immune modulating function and application thereof
  • Preparation method of Jinchang jujube polysaccharide with immune modulating function and application thereof

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Embodiment Construction

[0034] The first part preparation of Jinchang jujube polysaccharide with immune regulation function

[0035] Example 1 Preparation of Jinchang jujube polysaccharide JCS-1

[0036] (1) Prepare Jinchang jujube defatted powder: fresh Jinchang jujube is pitted, dried and pulverized to obtain Jinchang jujube powder; then add 70 wt% ethanol with 10 times the quality of Jinchang jujube powder to Jinchang jujube powder, fully stir, and stand for 2 day, discard the ethanol solution in the upper layer, repeat the operation 3 times, and finally evaporate the ethanol to dryness to obtain the degreasing powder of Jinchang jujube;

[0037] (2) Carry out water extraction on the degreased powder of Jinchang jujube to obtain a water-extraction concentrate: add water to the degreased powder of Jinchang jujube, the ratio of solid to liquid between the defatted powder of Jinchang jujube and water is 1:10g / mL, extract at 80°C for 3 hours, and repeat the operation Three times, the water extracts...

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Abstract

The invention discloses a preparation method of Jinchang jujube polysaccharide with immune modulating functions and an application thereof. The preparation method comprises the following steps: (1) preparing Jinchang jujube degreased powder; (2) carrying out aqueous extraction on the Jinchang jujube degreased powder to obtain aqueous extraction concentrated liquor; (3) removing proteins in the aqueous extraction concentrated liquor to obtain de-protein concentrated liquor; (4) preparing Jinchang jujube coarse polysaccharide from the de-protein concentrated liquor by means of an alcohol deposition method; and (5) carrying out separation to obtain the Jinchang jujube coarse polysaccharide with the immune modulating functions. The Jinchang jujube polysaccharide prepared by the invention has the immune modulating functions of promoting phagocytosis of macrophage, increasing output of nitric oxide of macrophage, promoting proliferation of splenic lymphocyte of mice and the like.

Description

technical field [0001] The invention relates to the technical field of deep processing of natural polymer and Jinchang jujube. Specifically, it is a preparation method and application of Jinchang jujube polysaccharide with immune regulation function. Background technique [0002] Jujube is an excellent tonic of "medicine and food from the same source" in traditional Chinese medicine in my country. It has a wide range of applications and a long history of medicinal use. Its health care effect is well known to everyone. Jujube polysaccharide is an important natural active ingredient in jujube. It has obvious immune activity and promotes the proliferation of lymphocytes. It has important effects on anti-oxidation and anti-aging. It also has the functions of nourishing qi and blood, improving intestinal tract and anti-fatigue. . Therefore, it is necessary for us to conduct in-depth research and rational utilization of jujube polysaccharide resources. Its extraction method has ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P37/02
CPCA61P37/02C08B37/0003C08B37/0087Y02A50/30
Inventor 白红进蔡雨晴吴翠云陈朋石建敏
Owner TARIM UNIV
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