The invention relates to the technical field of Chinese herbal
medicine cultivation, in particular to a sterilized
processing method for
bletilla striata explants. The sterilized
processing method for the
bletilla striata explants comprises the following steps of digging, sand culturing and sterilized
processing. According to the sterilized processing method for the
bletilla striata explants, the artificial mechanical
tissue damage is small, and the integrity of
plant tissue is effectively guaranteed; in a tissue culturing process, compared with a traditional culturing, the
browning and the
vitrification of plants are obviously reduced, a large amount of economic loss caused by the
browning and the
vitrification is effectively saved, and the work efficiency in the field is obviously improved; with the increase of
tissue culture subculture times, the
plant individual genetic conditions are stable, and the value-added effect is obvious; since after the sterilized processing, the
infection rate of explant thalli is effectively controlled within 2.5%, which is obviously lower than 30-50% of a traditional
infection rate; after the processing of the steps, a premise guarantee is provided for artificial effective selecting strong and healthy
bletilla striata seed source. The root, the stem and leaves of the seeds at a tissue culturing later stage is strong and healthy, the seedball expands obviously, the
survival rate of
transplanting to large fields is above 95.67%, and the growing
momentum of the later stage is obvious.