Aseptic treatment method of bletilla striata explants

Abstract

The invention relates to the technical field of Chinese herbal medicine cultivation, in particular to a sterilized processing method for bletilla striata explants. The sterilized processing method for the bletilla striata explants comprises the following steps of digging, sand culturing and sterilized processing. According to the sterilized processing method for the bletilla striata explants, the artificial mechanical tissue damage is small, and the integrity of plant tissue is effectively guaranteed; in a tissue culturing process, compared with a traditional culturing, the browning and the vitrification of plants are obviously reduced, a large amount of economic loss caused by the browning and the vitrification is effectively saved, and the work efficiency in the field is obviously improved; with the increase of tissue culture subculture times, the plant individual genetic conditions are stable, and the value-added effect is obvious; since after the sterilized processing, the infection rate of explant thalli is effectively controlled within 2.5%, which is obviously lower than 30-50% of a traditional infection rate; after the processing of the steps, a premise guarantee is provided for artificial effective selecting strong and healthy bletilla striata seed source. The root, the stem and leaves of the seeds at a tissue culturing later stage is strong and healthy, the seedball expands obviously, the survival rate of transplanting to large fields is above 95.67%, and the growing momentum of the later stage is obvious.

Description

technical field [0001] The invention relates to the technical field of Chinese herbal medicine cultivation, in particular to a method for aseptically treating bletilla striata explants. Background technique [0002] Bletilla striata [Betillastriata (Thunb.) Reichb.f.] belongs to Orchidaceae and belongs to perennial herb. It is one of the folk traditional Chinese medicinal materials in my country. The market demand is large, and in the natural state, reproduction is difficult. In recent years, artificial over-harvesting and destruction of the natural ecological environment have sharply reduced wild Bletilla striata resources and are on the verge of extinction. Bletilla striata has been listed as one of the key protected wild medicinal plants in China. Baiji has the functions of astringent and hemostasis, detumescence and granulation, and can treat diseases such as hemoptysis and hematemesis, pulmonary tuberculosis and hematemesis, pertussis, and mucosal injury after radiot...

AI Technical Summary

Problems solved by technology

[0004] To sum up, whether it is a tuber or a seed capsule, the aseptic treatment generally goes through the process of "cleaning-alcohol soaking-0.1% disinfection-cleaning". Bacteria effect is at 60~70% (but according to the current research, the hereditary traits of Bletilla striata seed group culture are unstable, and the rate of seedling fall in field cultivation of seedlings is as high as more than 80%); , HgCl and other highly irritating disinfectants, to a certain extent, have brought secondary damage to the sterilized exosomes, although they can achieve about 30-50% aseptic effect, but with the increase of disinfection time, the operation Different personnel, differences in reagent concentrations, different sampling sites, and increased cleaning times will cause devastating damage to the internal tissue cells of the bulbs and lateral buds. Even the surviving lateral buds will be subject to a longer culture period leading to severe browning and eventually death

It brings irreparable huge economic losses to scientific research and production, and seriously hinders the development and research in this field.