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Tissue culture reproduction method for liquidambar styraciflua

A technology of tissue culture and red maple, which is applied in the field of plant reproduction, can solve the problems of large reproduction coefficient, low rooting rate, slow cultivation and growth, etc., and achieve the effects of high quality, improved survival rate, and good value-added effect

Inactive Publication Date: 2012-10-10
吴昌海
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AI Technical Summary

Problems solved by technology

However, although the method of sexual reproduction using seeds is simple, the reproduction coefficient is large and the growth cycle is long, which is not conducive to the promotion and production of red maple.
Grafting or cutting propagation is used in asexual reproduction. Although the seedlings are produced quickly and the seedlings are formed quickly, the rooting rate is always low and the root system is poor. Even if it survives, the later cultivation and growth are still slow

Method used

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Experimental program
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Embodiment Construction

[0013] The tender stems with 3-6 buds of American red maple were used as explants, cut into sections after disinfection, and inoculated into the medium of MS+BA+NAA, and the tissue induction culture was carried out under suitable conditions, and the ratio of light-dark time was 3:1.

[0014] The invention adopts the medium combination of MS+BA+NAA, plus a certain amount of agar and sucrose, and meets the conditions of tissue culture in terms of inorganic matter, nitrogen source, carbon source, and auxin.

[0015] The medium is MS+2.0mg / L 6-BA+0.2mg / L NAA medium, and 20~25g sucrose and 10~15g agar are added to each liter of medium. The culture bacteria were sterilized by high-pressure steam at 121 degrees Celsius for 20 minutes before adding the curing agent. Wherein NAA is added after steam sterilization.

[0016] The explants are the tender stems of healthy American red maple with 3-6 buds as the explants.

[0017] The disinfection method of explants is: rinse the explants...

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Abstract

The invention discloses a tissue culture reproduction method for liquidambar styraciflua. The method comprises the following steps: taking a tender stem of liquidambar styraciflua provided with 3-6 buds as an explants, sterilizing, segmenting, inoculating into an MS+BA+NAA culture medium, performing tissue induction culture under suitable conditions, and ensuring that the time ratio of radiation to dark is 3:1. The scientific culture medium is established and assisted with a reasonable technical means, and tissue culture reproduction with good multiplication effect and high quality is realized. When the culture medium is used for culturing the explants, the browning of the explants is avoided, so that the survival rate of tissues is improved.

Description

technical field [0001] The invention relates to the field of plant propagation, in particular to a tissue culture propagation method of American red maple. Background technique [0002] American red maple, also known as red maple, northern red maple, North American red maple, swamp maple, Canadian red maple, brown sugar maple, scarlet maple, is native to the northeastern United States. It has beautiful tree posture, luxuriant branches and leaves, slender leaf shape, beautiful leaf color, and the earliest color change in autumn. The main color is bright red, the secondary color is orange, and the color is as bright as flowers. It is mainly used for garden viewing and garden greening. [0003] The American red maple, like other colorful plants, can reproduce sexually or asexually. However, although the method of sexual reproduction by seed propagation is simple, the reproduction coefficient is large and the growth cycle is long, which is not conducive to the popularization an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 吴昌海
Owner 吴昌海
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