Serum-free culture system capable of promoting induced pluripotent stem cells to be differentiated to cortical nerve cells

Abstract

The invention belongs to the technical field of stem cell biology and cell culture and provides a serum-free culture system capable of promoting induced pluripotent stem cells to be differentiated tocortical nerve cells. The serum-free culture system specifically comprises a serum-free differentiation basic culture medium and additives capable of promoting cortical nerve cell differentiation, andnecessary ingredients of the serum-free basic culture medium include DMEM / F12 culture medium, human serum albumin, L-glutamine, D-glucose, vitamin C, N2 / B27 cell culture additive and non-essential amino acid. The additives capable of promoting cortical nerve cell differentiation specifically include SB431542, dermorphin, fibroblast growth factor-2, retinoic acid, BDNF, GDNF, CTNF, DAPT and cytarabine. By using the culture medium, cortical nerve cell differentiation in a cell mixed system can be promoted, and differentiation towards cells of other types is reduced, so that high-purity corticalnerve cells are obtained finally.

Description

technical field [0001] The invention belongs to the technical fields of stem cell biology and cell culture, and specifically relates to a serum-free culture system for promoting the differentiation of induced pluripotent stem cells into cortical nerve cells. Background technique [0002] Neurodegenerative diseases are a kind of diseases with slow onset, progressive development and poor prognosis. So far, there is no effective cure method. Common clinical neurodegenerative diseases include Parkinson's disease, amyotrophic lateral sclerosis, spinal muscular atrophy, and Alzheimer's disease. Although these diseases have different etiologies, they all have different parts of the central nervous system and different degrees of neuron loss and dysfunction in pathology. Replacing lost neurons and restoring their function is a new approach to treating these diseases. Studies in recent years have found that stem cells cultured in vitro can not only proliferate and differentiate, bu...

AI Technical Summary

Problems solved by technology

[0004] Most of the medium for pluripotent stem cell culture and differentiation into nerve cells contains fetal bovine serum components. The components of serum are unknown and complex. Due to the large differences in batches of serum products from different manufacturers, the repeatability of the experiment is extremely low.

In addition, serum may also carry pathogens and other pathogenic factors, which greatly reduces the application value and safety of differentiated nerve cells in drug screening testing or cell therapy.

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