Method for preparing cytoplasmic male sterile hybrid varieties for improving rapeseed planting purity

Abstract

The invention belongs to the technical field of rapeseed breeding, and in particular, relates to a method for preparing cytoplasmic male sterile hybrid varieties for improving rapeseed planting purity, wherein the method includes the steps: obtaining of a recessive new leaf yellowing mutant of rapeseed; cultivation of a new leaf yellowing cytoplasmic male sterile maintainer line of the rapeseed; transformation of a new leaf yellowing cytoplasmic male sterile line of the rapeseed; hybridization of the new leaf yellowing cytoplasmic male sterile line and a non-yellowing leaf color cytoplasmic male sterile restorer line of the rapeseed to prepare a hybrid variety, that is to say, preparation of the hybrid variety with the new leaf yellowing cytoplasmic male sterile line of the rapeseed as a female parent, a green leaf color or other non-yellowing leaf color cytoplasmic male sterile restorer line as a male parent; and removal of new leaf yellowing seedlings in the hybrid variety, so as toimprove the planting purity of the rapeseed hybrid varieties. The high and stable yield of the hybrid rapeseed varieties can be ensured; compared with traditional methods for preparing cytoplasmic male sterile hybrid rapeseed varieties, the method can simplify variety preparing procedures, reduce variety production risk, increase variety production yield, greatly reduce labor and cost and avoid environmental pollution.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, in particular to a method for preparing cytoplasmic male sterile hybrid varieties for improving the planting purity of rapeseed. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: China is a big producer and consumer of rapeseed, ranking first in the world in terms of planting area, total output and consumption. Rapeseed production plays an important role in our national economy and national life. Breeding of high-yielding and stable-yielding rapeseed varieties is the fundamental guarantee for the safety of rapeseed production. The use of heterosis is an important way to increase the yield of rapeseed. Heterosis refers to the phenomenon that biological hybrids are superior to their parents in terms of growth vigor, vitality, stress resistance, yield and quality. Rapeseed rape has strong heterosis, and its hybrids can usua...

AI Technical Summary

Problems solved by technology

[0006] At present, there is no ideal measure to completely overcome the trace amount of fertile pollen of cytoplasmic male sterile lines such as Rapeseed Polyma and Shan 2A, and the selfed seeds of cytoplasmic male sterile plants are always mixed in the cytoplasmic male sterile hybrid seeds, thereby reducing the chance of hybridization. The purity of the variety promotion and planting prevents the heterosis from being fully realized, resulting in a decrease in yield

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