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Breeding method for rice-blast-resistant borer-resistant transgenic rice sterile line

A technology of rice blast resistance gene and transgenic rice is applied in the direction of plant gene improvement, botanical equipment and method, application, etc., which can solve the problems of restricting the production capacity of rice, and achieve the effects of stable fertility, improved work efficiency and low temperature.

Inactive Publication Date: 2018-12-07
INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the problem of rice production capacity restricted by rice diseases and insect pests in the prior art, and to provide a multi-objective trait aggregation breeding method for transgenic rice male sterile lines

Method used

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Experimental program
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Effect test

Embodiment 1

[0064] 1. In the spring of the first year in Hainan, the male sterile line E Nong 1S carrying the endogenous rice blast resistance genes Pi-1 and Pi-2 was used as the female parent, and a rice homozygous line with the non-selectable marker-transferred insect resistance gene Cry1Ab mfb-MH86 is the male parent, and the two are crossed to obtain F 1 seed;

[0065] 2. Planting F in Haiwuhan in the same year 1 Seed gets F 1 group, and then backcross with Enong 1S to obtain BC 1 f 1 Seeds, BC planted in Hainan in the second spring 1 f 1 to get BC 1 f 1 groups, then to BC 1 f 1 The leaves of the population were taken plant by plant to detect the Cry1Ab protein test strips, and the plants with the expression of the insect resistance gene Cry1Ab were selected as the male parents for backcrossing with Enong 1S to obtain BC 2 f 1 Seeds, wherein the detection of Cry1Ab protein test strips is as follows: first take 1-2cm paddy rice seedling stage or mature stage leaves and place...

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Abstract

The invention discloses a breeding method for a rice-blast-resistant borer-resistant transgenic rice sterile line. According to the method, a sterile line E Nong 1S carrying rice endogenous rice-blast-resistant genes Pi-1 and Pi-2 is taken as a female parent, a rice homozygous line mfb-MH86 with selective marker-free transform insect-resistant genes Cry1Ab is taken as a male parent, Cry1Ab proteintest strip detection, molecular detection, fertility identification and agronomic trait investigation are combined by virtue of a breeding manner of hybridizing first and sequentially backcrossing isperformed for three times. Therefore, new rice varieties having low initial fertility temperature and stable fertility and polymerizing rice blast resistance and borer resistance are screened, high accuracy and high reliability of the selection results are ensured, and the working efficiency for pyramiding breeding of the transgenic insect-resistant rice sterile line is improved.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a breeding method for rice blast-resistant and borer-resistant transgenic sterile lines of rice. Background technique [0002] Rice is the largest food crop in my country. The annual sowing area of ​​rice is about 30 million hectares, and the total harvest accounts for about 40% of my country's total grain output. Rice production is the mainstay of ensuring national food security. The rice varieties currently used in production are poor in resistance to diseases and insect pests, and are susceptible to damage from diseases and insect pests, resulting in reduced yield and unstable quality performance of rice. It is an important measure of the comprehensive ability of disease, insect resistance and yield. [0003] Gene aggregation refers to the aggregation of target genes scattered in different varieties into one variety. Through this method, it is possible to b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 殷得所査中萍戚华雄万丙良杜雪树李进波夏明元
Owner INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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