Sperm freezing method based on ICSI micromanipulation

Abstract

The invention belongs to the technical field of medicines and relates to a sperm freezing method based on ICSI micromanipulation. The method comprises the following steps: (1) preparing two ICSI dishes, introducing sperm samples into one ICSI dish, and introducing one or multiple drops of sperm freezing protecting liquid into the other ICSI dish; (2) extracting motile sperms from the ICSI dish containing the sperm samples by virtue of a single sperm injection needle, and introducing the motile sperms into liquid drops in the sperm freezing protecting liquid in the other ICSI dish; and (3) putting the ICSI dish containing the liquid drops of the sperm freezing protecting liquid into liquid nitrogen, freezing, and carrying out preservation. The method has the beneficial effects that a carrier is saved, the intermediate links of a small amount of sperms in the freezing and unfreezing processes are remarkably reduced, the activity ratio of the unfrozen sperms is high, the loss rate is low,and the utilization rate of the sperms is high.

Description

technical field [0001] The invention belongs to the field of medical technology, and more specifically relates to a method for freezing semen based on ICSI micromanipulation. Background technique [0002] Frozen sperm is a necessary technology in assisted reproduction. During the process of in vitro fertilization, some patients need to freeze their sperm in advance for in vitro fertilization due to reasons such as a small number of motile sperm or difficulty in obtaining sperm. The number of motile sperm in some patients is small, and there are many procedures for freezing in conventional methods. During the operation, a variety of instruments are in contact with the sperm to be frozen or thawed and their cryoprotectants, which may easily lead to a small amount of sperm to be frozen or thawed. Lost and difficult to find motile sperm after thawing. In clinical work, if the number of motile sperm is less than 10-15 before freezing, it will be difficult to find motile sperm af...

AI Technical Summary

Problems solved by technology

CN108207931A discloses a micro-sperm cryopreservation method, wherein although no straw, capsule or zona pellucida is used, hydrogel microspheres are still used as the carrier, and the sperm and its protective agent need to be sucked out from the carrier when thawing to make Only liquid droplets can be used, adding too many micromanipulations and intermediate links

[0005] Therefore, the existing technology of freezing and semen for a small amount of sperm has many operating links. During the operation, various instruments are in contact with the sperm to be frozen or thawed and their cryoprotectants, which may easily lead to the loss of a small amount of sperm to be frozen or thawed, and after thawing It is necessary to transfer the sperm and its cryoprotectant to a certain container before use, which increases the intermediate link in the operation process and significantly increases the possibility of sperm loss and inactivation

At the same time, the use of carriers also increases the cost of purchasing carriers and increases the technical difficulty and operating time of operators.

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