A kind of high-density fermentation culture process of mycoplasma gallisepticum
A high-density fermentation technology of Mycoplasma gallisepticum, which is applied in the field of agricultural microorganisms, can solve the problems of Mycoplasma gallisepticum difficult to produce on a large scale, and achieve the effects of improving the protection effect, increasing the density of bacteria, and solving the difficulty of large-scale production
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Embodiment 1
[0022] A kind of Mycoplasma gallisepticum high-density fermentation culture technique provided by the present embodiment 1 comprises the following steps:
[0023] Step 1: Take the freeze-dried seeds of Mycoplasma gallisepticum for production and inoculate them in the culture medium of Mycoplasma gallisepticum at a ratio of 1:10, and culture them for 18-24 hours under the condition of 36-37°C to obtain the culture medium, and in the same way The obtained culture medium was inoculated and passaged again at a ratio of 1:10;
[0024] Step 2: Inoculate the bacterium liquid after the above-mentioned subculture into the sterilized Mycoplasma gallisepticum culture medium of a 20L fermenter at a ratio of 3% / V / V, and the cultivation process conditions of the fermenter are: the temperature is 37°C, and the tank pressure is 0.03Mpa, the dissolved oxygen is 40%, the pH value is 7.7, and the stirring speed is 100rpm. During the cultivation process, when adding 20% NaOH solution online to ...
Embodiment 2
[0030] A kind of Mycoplasma gallisepticum high-density fermentation culture process that present embodiment 2 provides, comprises the following steps:
[0031] Step 1: Take the freeze-dried seeds of Mycoplasma gallisepticum for production and inoculate them in the culture medium of Mycoplasma gallisepticum at a ratio of 1:10, and culture them for 18-24 hours under the condition of 36-37°C to obtain the culture medium, and in the same way The obtained culture medium was inoculated and passaged again at a ratio of 1:10;
[0032] Step 2: Inoculate the bacterium liquid after the above-mentioned subculture into the sterilized Mycoplasma gallisepticum culture medium of a 20L fermenter at a ratio of 4% / V / V, and the cultivation process conditions of the fermenter are: the temperature is 37°C, and the tank pressure is 0.05Mpa, the dissolved oxygen is 40%, the pH value is 7.7, and the stirring speed is 100rpm. During the cultivation process, when adding 20% NaOH solution online to con...
Embodiment 3
[0039] A kind of Mycoplasma gallisepticum high-density fermentation culture technique provided by the present embodiment 3 comprises the following steps:
[0040] Step 1: Take the freeze-dried seeds of Mycoplasma gallisepticum for production and inoculate them in the culture medium of Mycoplasma gallisepticum at a ratio of 1:10, and culture them for 18-24 hours under the condition of 36-37°C to obtain the culture medium, and in the same way The obtained culture medium was inoculated and passaged again at a ratio of 1:10;
[0041]Step 2: Inoculate the bacterium liquid after the above-mentioned subculture into the sterilized Mycoplasma gallisepticum culture medium of a 20L fermenter at a ratio of 3% / V / V, and the cultivation process conditions of the fermenter are: the temperature is 37°C, and the tank pressure is 0.03Mpa, the dissolved oxygen is 40%, the pH value is 7.7, and the stirring speed is 100rpm. During the cultivation process, when adding 20% NaOH solution online to c...
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