Cell cryopreservation box

A freezing box and cell technology, applied in the field of cell biology, can solve the problems of unenvironmental protection, complex structure of the cooling box, inconvenient use, etc., and achieve the effects of low cost, broad market prospects, application value, and convenient access.

Pending Publication Date: 2018-12-14
四川妙和生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is that the existing cell cryopreservation program cooling box has the defects of complex structure, inconvenient use and non-environmental protection, and an environmentally friendly and convenient cell cryopreservation box is provided

Method used

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  • Cell cryopreservation box
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  • Cell cryopreservation box

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Utilize three different cell freezing boxes (isopropanol freezing box, NEB freezing box, polyethylene foam freezing box of the present invention) to cryopreserve HEK293 cells (Wuwu Biological Company), the specific steps are as follows:

[0043] 1. Cell passage

[0044] 1.1 Take out the freezing liquid, PBS, Tripsn, and culture medium from the refrigerator. Place in a water bath to pre-warm.

[0045] 1.2 Take out the culture dish containing HEK293 cells in the incubator, observe under the microscope that the cell plating rate reaches 80% to 90%, use a waste liquid gun to suck out the waste liquid in the culture dish, and use an electric gun to take 3ml of PBS solution along the culture dish. Shake to wash the cells, and then aspirate the PBS waste solution.

[0046] 1.3 Take 1.5ml Tripsn and add it to the petri dish along the edge, shake gently to infiltrate the cells in the Tripsn. Observe under the microscope whether the cells are completely infiltrated, and put th...

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Abstract

The invention discloses a cell cryopreservation box. The cell cryopreservation box comprises a box body and a box cover. A plurality of first cryotube holes for storing cryotubes and a fixed hole arearranged in the box body. The height of the first cryotube holes is smaller than that of the cryotube. The fixed hole is arranged in the center of the box body. The box cover is provided with second cryotube holes corresponding to the first cryotube holes of the box body. The center of the box cover is provided with a fixed raised block corresponding to the fixed hole. The shape and size of the fixed raised block of the box cover are matched with those of the fixed hole of the box cover. The cell cryopreservation box has good heat preservation performances, slowly increases or decrease the temperature in the cryotube hole, guarantees the slow cooling of the cells in a refrigerator at -80 DEG C and ensures the high cell cryopreservation survival rate. The cell cryopreservation box is free of a cryopreservation liquid in the cryopreservation device, is environmentally friendly and has no pollution.

Description

technical field [0001] The invention relates to the technical field of cell biology, in particular to a special cryopreservation container for cell cryopreservation tubes. Background technique [0002] In the process of subculture and daily maintenance of cell culture, a lot of expense is required in terms of culture equipment, culture medium and various preparations, and once the cells leave the living body to start primary culture, its various biological characteristics will gradually change and become With the increase of the number of passages and the change of the environmental conditions in vitro, there are new changes. Therefore, timely cell cryopreservation is very necessary. Cell cryopreservation is an important part of cell culture. The key point of cryopreservation technology is slow freezing, and the standard freezing procedure is a cooling rate of -1 to -2°C / min; when the temperature falls below -25°C, it can be increased to -5°C to -10°C / min. Previously, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0268
Inventor 张茂祥章文裴毓武勇
Owner 四川妙和生物科技有限公司
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