Method for separating and purifying functional proteins in plasma

Abstract

The invention discloses a method for separating and purifying functional proteins in plasma. The method includes the following steps: step S1 of liquid preparation: firstly, plasma to be separated andpurified is poured into a measuring cup, 50 ml of plasma is measured, and all the plasma is divided into a plurality of groups according to a unit of 50 ml, adding 50 ml of soluble inorganic salt inthe plasma solution, and stirring the solution for 20-30 min until the solution is stirred evenly, so that a plurality of groups of plasma salt solutions can be obtained. The invention relates to thetechnical field of plasma protein purification. According to the method for separating and purifying functional proteins in plasma, multi-group separation and purification of the plasma can be achieved, the goal of two-time elution of the plasma is achieved, so that separating and purifying effects are greatly enhanced, the functional proteins in the plasma are quickly and efficiently separated and purified, the purity of the functional proteins can be improved to a higher degree, and the goal of facilitating the purification of the functional proteins is achieved, thereby greatly facilitatingusers' use of the functional proteins.

Description

technical field [0001] The invention relates to the technical field of plasma protein purification, in particular to a method for separating and purifying functional proteins in plasma. Background technique [0002] Protein is a macromolecular substance, and different proteins have different molecular sizes. Therefore, some simpler methods can be used to separate proteins from small molecular substances, and to separate protein mixtures. The method of separation according to the different sizes of protein molecules is mainly There are dialysis, ultrafiltration, centrifugation and gel filtration, etc. Dialysis and ultrafiltration are commonly used methods for separating proteins. Dialysis is to put the mixture to be separated into a dialysis bag made of a semi-permeable membrane, and then immerse it in the dialysate for separation. Ultrafiltration is the process of using centrifugal force or pressure to force water and other small molecules to pass through a semi-permeable me...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the present invention provides a method for separating and purifying functional proteins in plasma, which solves the problem that the separation and purification methods for separating and purifying functional proteins in plasma have poor separation and purification effects and cannot realize the separation and purification of functional proteins. The functional protein in the plasma is separated and purified quickly and efficiently, and the purity of the functional protein separation cannot be extracted to a high degree, and the purpose of facilitating the purification of the functional protein cannot be achieved, thus bringing people the use of the functional protein. great inconvenience