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Treatment method for simulating drought stress of Rhododendron simsii

A technology of drought stress and treatment method, applied in the field of plant breeding, can solve the problem that the root system of Rhododendron is not easy to be treated with drought, and achieve the effects of saving preliminary exploration work, high practical value and convenient operation.

Inactive Publication Date: 2019-01-04
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a treatment method for simulating the drought stress of Rhododendron, save the preliminary work of exploring the specific conditions of Rhododendron simulated drought, and solve the problem that the root system of Rhododendron is too developed and it is difficult to use traditional hydroponic devices for drought treatment.

Method used

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  • Treatment method for simulating drought stress of Rhododendron simsii
  • Treatment method for simulating drought stress of Rhododendron simsii
  • Treatment method for simulating drought stress of Rhododendron simsii

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Embodiment 1

[0021] 1) Select rhododendrons with the same seedling age and growth, wash their roots with clean water and disinfect them, put them in nutrient solution (as shown in Table 1) for adaptive cultivation, and simulate drought stress after the adaptive cultivation of rhododendrons is completed;

[0022] Table 1 Nutrient solution formula

[0023]

[0024] 2) Polyethylene glycol 6000 was used as a water stress agent, and 78.5 g, 178.4 g, and 261.9 g of polyethylene glycol 6000 were added to 1 L of nutrient solution to simulate the drought conditions of -0.1 MPa, -0.4 MPa, and -0.8 MPa, Set the ambient temperature to 25°C, as shown in Table 2;

[0025] Table 2 Osmotic potentials corresponding to different concentrations of polyethylene glycol 6000 solutions at 25°C

[0026]

[0027] 3) Choose a round plastic box with a lid with a capacity of 1 L as the container;

[0028] 4) Cut the circular plastic cover along any radius, and cut off a triangle with a side length of 1 cm in ...

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Abstract

The invention discloses a treatment method for simulating drought stress of Rhododendron simsii. Rhododendron simsii seedlings with a same seedling age are selected, hypertonic solutions with different osmotic potentials are used for stress treatment, the ambient temperature is 25 DEG C, a round plastic box with a lid is used as a container, the center part of the plastic lid is cut off, Rhododendron simsii is placed in the center part, the stem is fixed by a sponge, the root system is immersed in a hypertonic solution, and after 36 hours of stress treatment, relevant physiological indexes aredetermined. The method of the invention not only saves preliminary work of exploring specific conditions of simulating drought for Rhododendron simsii, but also solves the problem that the roots of Rhododendron simsii are too developed and cannot easily use a traditional hydroponic device for drought treatment, and the treatment method achieves the purpose of convenient and quick treatment.

Description

technical field [0001] The invention belongs to the field of plant breeding, and in particular relates to a treatment method for simulating drought stress of Rhododendron. Background technique [0002] The traditional method of simulating the drought stress of plant roots is to plant the plants in containers with the same solid substrate composition, to achieve a certain degree of stress by controlling the amount of watering, or to wait for it to dry slowly after watering enough at one time. This method is easy to operate, but there is a situation that the moisture content between the upper matrix and the lower matrix in the container is inconsistent, the time is long, and the solid matrix is ​​difficult to have high uniformity, resulting in difficult control of the water content, which will affect the final test. result. The simulated root drought conditions can be used to study the physiological and biochemical changes of Rhododendron under drought conditions, which can p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01G7/06
CPCA01G7/06A01G31/00
Inventor 陈清西郑颖王威谢倩
Owner FUJIAN AGRI & FORESTRY UNIV
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