Application of Cationic Polyvinylamine Linear Polymer as Transgenic Carrier

A technology of transgenic carrier and polyvinylamine, which is applied in the fields of polymer chemistry and biological materials, can solve the problems of low transfection efficiency, high price, and restrictions on the application of polycationic polymers, and achieve high transfection efficiency, low price, The effect of good biocompatibility

Active Publication Date: 2021-07-20
西安九清医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently commercially available polycationic polymers that can be used for nucleic acid delivery are expensive and have low transfection efficiency, which greatly limits the application of polycationic polymers.

Method used

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  • Application of Cationic Polyvinylamine Linear Polymer as Transgenic Carrier
  • Application of Cationic Polyvinylamine Linear Polymer as Transgenic Carrier
  • Application of Cationic Polyvinylamine Linear Polymer as Transgenic Carrier

Examples

Experimental program
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Effect test

example 1

[0036] A cationic polyvinylamine linear macromolecule is used as a transgenic carrier, and the structural formula (I) of the linear macromolecule transgenic carrier is as follows:

[0037]

[0038] In formula (I), x / (x+y)=0.5-1. Its raw material trade name is XELOREX TM RS 1100.

[0039] For the linear macromolecular transgene carrier raw material (XELOREX TM RS 1100) has been purified as follows:

[0040] With water as the dialysis medium, XELOREX TM The RS 1100 aqueous solution (concentration is 10%) is dialyzed for 72 hours through MWCO8-14kd dialysis bag (molecular weight permeation <10000) to remove small molecules in the raw material that are toxic to cells. After dialysis, it was freeze-dried. The obtained powder was redissolved with triple distilled water, and prepared as a stock solution of appropriate concentration for use.

[0041] The linear polymer transgene carrier in Example 1 is tested in the following aspects:

[0042] (1) Agarose gel electrophore...

example 2

[0072] A cationic polyvinylamine linear macromolecule is used as a transgenic carrier, and the structural formula (I) of the linear macromolecule transgenic carrier is as follows:

[0073]

[0074] In formula (I), x / (x+y)=0.5-1. Its raw material trade name is XELOREX TM RS 1100.

[0075] For the linear macromolecular transgene carrier raw material (XELOREX TM RS 1100) has been purified as follows:

[0076] With water as the dialysis medium, XELOREX TM The RS 1100 aqueous solution (concentration is 10%) is dialyzed for 72 hours through MWCO8-14kd dialysis bag (molecular weight permeation <10000) to remove small molecules in the raw material that are toxic to cells. After dialysis, it was freeze-dried. The obtained powder was redissolved with triple distilled water, and prepared as a stock solution of appropriate concentration for use.

[0077] The linear polymer transgene carrier in Example 2 is tested in the following aspects:

[0078] (1) Transgenic carrier cytot...

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Abstract

The invention provides a cationic polyvinylamine linear polymer transgene carrier whose trade name is XELOREX TM RS 1100. The invention provides the application of the linear polymer transgene carrier as a nucleic acid molecule delivery carrier. The invention achieves high-efficiency transfection effect in the process of cell transfection. The cost of materials is low, the cytotoxicity of the transfection process is low, and gene molecules can be effectively and safely delivered to cells. The linear polymer transgene carrier of the present invention has the advantages of high efficiency, low toxicity and low price.

Description

technical field [0001] The invention belongs to the technical field of polymer chemistry and biomaterials, and in particular relates to the application of a cationic polyvinylamine linear polymer. Background technique [0002] As a new type of treatment, gene therapy has shown great application potential in the treatment of major diseases such as cancer, AIDS, and osteoporosis. However, due to the lack of safe and efficient gene carriers, gene therapy has not yet made substantial progress in clinical practice. [0003] Gene therapy refers to the introduction of exogenous normal genes into target cells by means of gene carriers to correct or compensate diseases caused by gene defects or abnormalities, so as to achieve the purpose of treatment. However, naked DNA generally exists in the form of a stretched linear helix with a loose volume, and the DNA molecule itself is a negatively charged anion, which will generate electrostatic repulsion with the anion on the outer layer o...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N15/87
CPCC12N15/87
Inventor邓旭东田野尹大川骞爱荣高永光王雪李瑞云张托弟
Owner西安九清医疗科技有限公司