A Single Cell Sorting System Based on Image Processing Model

Abstract

The invention discloses a single cell sorting device and method based on an image processing model. The device includes a controller, a sample chip, an industrial computer, a microscope, etc., and does not require tedious and repeated manual adjustment and selection of target cells, but uses The image processing algorithm cooperates with the high-precision motion system to complete the precise positioning of cells, avoiding manual positioning errors, time consumption and pollution, and can locate multiple single cells at one time, fast and high-throughput. In this method, the cell sample image collected on the sample chip is transferred to the industrial computer as input data, and the industrial computer uses the image processing model to obtain the image position information of each individual cell on the image, and then converts the image position information into the motion of the electric stage The position information realizes the rapid batch conversion from the cell group image to the cell group location, does not need to mark the cells, avoids the damage or state change of the cells caused by other sorting methods, and can obtain the real information of the cells.

Description

technical field [0001] The invention relates to the technical field of single cell sorting, in particular to a single cell sorting system based on an image processing model. Background technique [0002] Cells are the basic unit of life structure and life activities, and the analysis techniques of cell populations used in the past have many disadvantages and limitations. The analysis of cell populations or cell groups can only obtain the average signal of the cell population, but there are large differences between individual cells. The analysis of cell populations will cover up the individual differences between single cells, causing research in medicine, biology and other disciplines. obstacle. Only by studying single cells can comprehensive and accurate cell information be obtained. High-throughput, automated single-cell sorting technology has become the focus of single-cell research. [0003] Currently commonly used single cell sorting techniques mainly include fluore...

AI Technical Summary

Problems solved by technology

Laser capture microdissection is generally used for large-area cell separation. It has low precision, is difficult to achieve high throughput, and is easy to cause mechanical damage to cells during cutting.

Although fluorescence-activated cell sorting can be used as an effective means of separating cells, this method requires the addition of fluorescent labels to cells, which changes the in situ state of cells and sometimes affects cell viability.

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