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Cell normalized IOD value calculation method for DNA ploidy analysis device

A technology of analysis equipment and calculation method, which is applied in the field of cell normalized IOD value calculation, can solve problems such as errors, and achieve the effect of accurate IOD value

Active Publication Date: 2019-01-15
湖南品胜生物技术有限公司
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  • Description
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  • Application Information

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Problems solved by technology

[0005] The problem is that due to individual differences, equipment differences, staining differences, etc., the distribution of diploid nuclei IOD values ​​is not a simple unimodal situation, and there are actually multi-peak and flat-top situations, and the maximum value algorithm is only applicable to unimodal situations. , used in multi-peak and flat-top situations may cause large errors

Method used

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  • Cell normalized IOD value calculation method for DNA ploidy analysis device
  • Cell normalized IOD value calculation method for DNA ploidy analysis device
  • Cell normalized IOD value calculation method for DNA ploidy analysis device

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Embodiment Construction

[0022] The present invention will be described in detail below with reference to the drawings in the embodiments of the present invention.

[0023] combine Figure 1 to Figure 4 As shown, the method for calculating the normalized IOD value of a DNA ploidy analysis device disclosed in the present invention, in order to facilitate the normalization of the IOD value of the diploid cell nucleus in the case of multi-peak or flat top, is more accurate, The specific operation process of the calculation method of the normalized IOD value of the diploid nuclei is as follows:

[0024] 1. Sort the IOD values ​​of all cells in ascending order;

[0025] 2. Select cells that are less than the maximum IOD value of the 2-ploid cell and greater than the minimum IOD value of the 2-ploid cell;

[0026] 3. Calculate the number of cells per unit IOD;

[0027] 4. Take the number of cells per unit as the ordinate, and use the IOD as the horizontal bar to make a scatter diagram;

[0028] 5. Fitti...

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Abstract

The invention provides a cell normalized IOD value calculation method for a DNA ploidy analysis device. According to the method, cell nucleuses are allocated into consecutive intervals according to the difference of IOD values; the number of cell nucleuses in each interval is calculated; Gaussian curve fitting is performed, and a mu value is solved; the mu value is adopted as a diploid cell nucleus normalized IOD value; and therefore, it can be ensured that the diploid cell nucleus normalized IOD value is more accurate under multimodal and flat-top conditions.

Description

technical field [0001] The invention relates to a method for calculating a cell normalized IOD value of a DNA ploid analysis device. Background technique [0002] In recent years, malignant tumors have been at a high incidence in my country. According to statistics, about 7 people are diagnosed with malignant tumors every minute in our country. This has become the most important cause of fatality rate in our country, which has aroused great concern of governments at all levels. Taking cervical cancer as an example, it is the third most common malignant tumor in women in the world and the second most common malignant tumor in Chinese women. According to the estimates of the World Health Organization (WHO), there are more than 470,000 new cases of cervical cancer in the world every year, and China accounts for 28%. Early diagnosis and early treatment is an effective way to deal with the high incidence of cancer. Pathologists collect human exfoliated cells, including cervica...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B40/00
Inventor 贺权源刘朝前罗军
Owner 湖南品胜生物技术有限公司