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A method for calculating cell normalized iod value of dna ploidy analysis equipment

A technology of analysis equipment and calculation method, which is applied in the field of cell normalized IOD value calculation, can solve problems such as errors, and achieve the effect of accurate IOD value

Active Publication Date: 2021-12-21
湖南品胜生物技术有限公司
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The problem is that due to individual differences, equipment differences, staining differences, etc., the distribution of diploid nuclei IOD values ​​is not a simple unimodal situation, and there are actually multi-peak and flat-top situations, and the maximum value algorithm is only applicable to unimodal situations. , used in multi-peak and flat-top situations may cause large errors

Method used

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  • A method for calculating cell normalized iod value of dna ploidy analysis equipment
  • A method for calculating cell normalized iod value of dna ploidy analysis equipment
  • A method for calculating cell normalized iod value of dna ploidy analysis equipment

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Embodiment Construction

[0022] The present invention will be described in detail below with reference to the drawings in the embodiments of the present invention.

[0023] to combine Figure 1 to Figure 4 As shown, the method for calculating the normalized IOD value of a DNA ploidy analysis device disclosed in the present invention, in order to facilitate the normalization of the IOD value of the diploid cell nucleus in the case of multi-peak or flat top, is more accurate, The specific operation process of the calculation method of the normalized IOD value of the diploid nuclei is as follows:

[0024] 1. Sort the IOD values ​​of all cells in ascending order;

[0025] 2. Select cells that are less than the maximum IOD value of the 2-ploid cell and greater than the minimum IOD value of the 2-ploid cell;

[0026] 3. Calculate the number of cells per unit IOD;

[0027] 4. Take the number of cells per unit as the ordinate, and use the IOD as the horizontal bar to make a scatter diagram;

[0028] 5. Fi...

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Abstract

The method for calculating the cell normalized IOD value of a DNA ploidy analysis device in the present invention divides the cell nuclei into continuous intervals according to the size of the IOD value, calculates the number of cells in each interval, and adopts a Gaussian curve Fitting, calculate the μ value, and use the μ value as the normalized IOD value of the diploid cell nucleus to ensure that the normalized IOD value of the diploid cell nucleus is more accurate in the case of multi-peak and flat top.

Description

technical field [0001] The invention relates to a method for calculating a cell normalized IOD value of a DNA ploid analysis device. Background technique [0002] In recent years, malignant tumors have been at a high incidence in my country. According to statistics, about 7 people are diagnosed with malignant tumors every minute in our country. This has become the most important cause of fatality rate in our country, which has aroused great concern of governments at all levels. Taking cervical cancer as an example, it is the third most common malignant tumor in women in the world and the second most common malignant tumor in Chinese women. According to the estimates of the World Health Organization (WHO), there are more than 470,000 new cases of cervical cancer in the world every year, and China accounts for 28%. Early diagnosis and early treatment is an effective way to deal with the high incidence of cancer. Pathologists collect human exfoliated cells, including cervica...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G16B40/00
Inventor 贺权源刘朝前罗军
Owner 湖南品胜生物技术有限公司