Method for negatively screening cancer cell by using biomagnetic beads in liquid biopsy
A negative screening and liquid biopsy technology, applied in cell dissociation methods, biochemical equipment and methods, animal cells, etc., can solve problems such as interference in telomerase activity detection
Inactive Publication Date: 2019-01-25
江苏凯强医学检验有限公司
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[0007] Aiming at the deficiencies of the prior art, the present invention provides a method for negatively screening cancer cells by using biomagnetic beads in liquid biopsy, which solves the problem of interference of some immune cells in the detection of telomerase activity in body fluid samples
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[0022] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the protection scope of the present invention.
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The invention discloses a method for negatively screening cancer cells by using biomagnetic beads in liquid biopsy, which comprises the following steps: (a) centrifuging a sample through a density gradient to process the sample, and the sample is taken from the body fluid of a cancer patient to detect the processed sample, that is a candidate sample, and relates to the technical field of biological detection. The method utilizes biomagnetic beads to negatively screen cancer cells in liquid biopsy, two kinds of biomagnetic beads were used to adsorb the immune cells which could affect the detection of telomerase activity, so that the false positive results could not be found in the experiment, and other cells were still in the sample through negative screening, so that the false negative results could not be found in the experiment.
Description
Technical field [0001] The invention relates to the technical field of biological detection, in particular to a method for negatively screening cancer cells using biological magnetic beads in liquid biopsy. Background technique [0002] Cancer cells are produced by malignant transformation of normal cells and have uncontrollable proliferation and metastasis capabilities. Because cancer cells have high metastasis characteristics, they contain shed cancer cells in urine, blood and other body fluids of cancer patients, which can be used as indicators for early cancer screening and prognostic diagnosis. It is known that telomerase can be detected in more than 90% of cancer cells, while telomerase cannot be detected in normal somatic cells. Therefore, the detection of telomerase in cells in fresh body fluid samples can serve as a reference for early cancer screening and prognosis. As a non-invasive inspection method, it is of great significance in the early diagnosis of tumors. With...
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IPC IPC(8): C12N5/09
CPCC12N5/0693C12N2509/00
Inventor 顾凯强葛远龙李国庆杨奇飞黄洪斌周君
Owner 江苏凯强医学检验有限公司
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