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Coxsackie A16 virus IGA antibody quantum dot immunofluorescence chromatography test strip and kit

A chromatographic test strip and immunofluorescence technology, applied in the field of microbial detection, can solve the problems of fear of blood collection, interfere with the work of medical staff, etc., and achieve the effects of easy control, avoidance of spread and high fluorescence intensity

Active Publication Date: 2022-04-26
GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, many children are naturally afraid of blood collection, and resisting behavior during the blood collection process will interfere with the work of medical staff

Method used

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  • Coxsackie A16 virus IGA antibody quantum dot immunofluorescence chromatography test strip and kit
  • Coxsackie A16 virus IGA antibody quantum dot immunofluorescence chromatography test strip and kit
  • Coxsackie A16 virus IGA antibody quantum dot immunofluorescence chromatography test strip and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Preparation of Coxsackie A16 virus IgA antibody quantum dot immunofluorescence chromatography test strip

[0031] 1. Preparation of quantum dot particle labeling pad

[0032]Take 10-50 μl of water-soluble CdTe / ZnSe core-shell quantum dots (surface carboxyl modification, Beijing Zhongke Wuyuan Biotechnology Co., Ltd., article number W-3006-570), centrifuge at 6000rpm for 5-10min, add 100μl 10mM PBS (pH 7.2) Dissolved to obtain a quantum dot solution. Add 10-50 μl of 2% (mass concentration) N-hydroxysuccinimide and 2% (mass concentration) 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride to the quantum Add 100-1500 μl of goat anti-human IgA antibody (Sigma, Cat. No. 10884) solution at a concentration of 5 mg / ml to the spot solution, and stir at room temperature for 1-6 hours. After the reaction is complete, centrifuge at 6000 rpm for 5-10 min to obtain a precipitate, and add 1% (mass volume concentration) BSA (bovine serum albumin) solution to the p...

Embodiment 2

[0041] Embodiment 2: Specimen test and result judgment

[0042] Put the cotton swab into the subject's mouth, collect saliva on the cheeks and under the tongue, put the collected cotton swab into the sample tube containing the sample diluent and squeeze it fully to obtain sample solution 8, suck 100-200μl The sample solution 8 is dropped on the sample pad 1 of the immunofluorescence chromatography test strip of the present invention along the direction A, and the sample solution 8 moves toward the absorbent paper along the chromatography direction B. Irradiate the test strip with the ultraviolet flashlight 9 for 20-30 minutes to observe the result. The wavelength range of ultraviolet light is 200-400nm.

[0043] The formula for the sample diluent is as follows:

[0044] 0.3% gelatin, 0.15% casein, 0.1% tryptone, 0.1% ProClin300, 10 mM PBS, pH 7.2.

[0045] figure 2 A schematic diagram of the detection process of the Coxsackie A16 virus IgA antibody quantum dot immunofluor...

Embodiment 3

[0047] Embodiment 3: Comparison of the results of the Coxsackie A16 virus IgA antibody quantum dot immunochromatography test strip and the fluorescent PCR method of the present invention

[0048] Screen 100 clinical patients with hand, foot and mouth disease, collect saliva samples and throat swab samples from the patients, and use the Coxsackie A16 virus IgA antibody quantum dot immunochromatography test strip diagnostic method and the Coxsackie virus A16 virus fluorescent PCR method respectively. The test results are shown in Table 1 below:

[0049] Table 1: The results of the comparison between the diagnostic method of quantum dot detection test strip and fluorescent PCR method for IgA antibody of Coxsackie virus A16 virus

[0050]

[0051] Thus, the Coxsackie A16 virus IgA antibody quantum dot immunochromatographic test strip of the present invention is compared with the fluorescent PCR method, the positive rate reaches 94.59%, and the specificity is 92.06%, which can m...

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Abstract

The invention discloses a Coxsackie A16 virus IgA antibody quantum dot immunofluorescence chromatography test strip. The Coxsackie A16 virus IgA antibody quantum dot immunofluorescence chromatographic paper strip of the present invention comprises a backboard and a sample pad arranged on the backboard, a quantum dot particle marker pad, a nitrocellulose membrane and an absorbent paper. The test strip of the present invention uses quantum dot immunofluorescence technology, and is based on the Coxsackie A16 virus IgA antibody in the saliva sample as the detection target, which can be highly sensitive, simple and fast to diagnose the early infection of the Coxsackie A16 virus, and is helpful To monitor the epidemic situation of hand, foot and mouth disease in time and prevent the spread of the epidemic.

Description

technical field [0001] The invention belongs to the field of microorganism detection, and specifically discloses a Coxsackie A16 virus IgA antibody quantum dot immunofluorescence chromatography test strip. Background technique [0002] Coxsackie A16 virus belongs to poliovirus, and EV71 virus is known as the main pathogen causing hand, foot and mouth disease. The main symptoms of Coxsackie A16 virus include headaches, loss of appetite, sore throat, mouth ulcers, blisters on hands and feet, etc. Sometimes it can also cause severe neurological illness, and some of them can cause death in infants and young children. In China, Coxsackie A16 virus is prevalent almost every year, and the prevalence rate is higher than that of EV71 virus. The epidemic outbreak time is mainly from May to June every year, and there will also be a small epidemic peak from September to October. The affected population is mainly children under the age of 5, and the places where the disease occurs are ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/52G01N33/533G01N33/577
CPCG01N33/526G01N33/533G01N33/577
Inventor 王长兵许甜甜朱冰
Owner GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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