Nucleic acid extraction all-in-one machine

Abstract

The invention provides a nucleic acid extraction all-in-one machine. The nucleic acid extraction all-in-one machine comprises a reaction strip, a pipette module and a PCR reaction module, wherein the reaction strip is provided with a sample adding groove, a lysate groove, a washing liquid groove, an eluent groove, a waste liquid groove, an extracting and mixing groove and PCR reaction grooves; the pipette module comprises a pipette, an up-and-down moving table, a transverse moving table and an air pump used for driving the pipette to suck and spit liquid; the pipette is installed on the up-and-down moving table in an up-and-down moving mode; the up-and-down moving table is installed on the transverse moving table in a horizontal moving mode; and the pipette is used for sucking and spitting the liquid in each of the reaction grooves. A user only needs to place a collected sample and a desired analysis result can be obtained through direct one-step operation; and in the sample extraction and reaction process, false positive results caused by mutual pollution between samples are avoided.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to an all-in-one nucleic acid extraction machine. Background technique [0002] The statements in this section only provide background information related to the present disclosure and may not constitute prior art. [0003] Nucleic acid detection is currently widely used in various fields such as disease control center, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial detection, food safety detection, animal husbandry and molecular biology research. Therefore, biological detection is getting more and more attention. Ordinary nucleic acid detection steps are generally virus nucleic acid extraction, and then sub-sample for nucleic acid PCR amplification detection, but this method has relatively high professional requirements for operation, and many steps are likely to cause cross-contamination. For non-professionals, it is ofte...

AI Technical Summary

Problems solved by technology

Ordinary nucleic acid detection steps are generally virus nucleic acid extraction, and then sub-sample for nucleic acid PCR amplification detection, but this method has relatively high professional requirements for operation, and many steps are likely to cause cross-contamination. For non-professionals, it is often easy to make mistakes or do not know how to do it. The need for proper detection

[0004] The existing all-in-one machine for nucleic acid extraction and detection is just an additional PCR detection module added to the machine for nucleic acid extraction. The method of nucleic acid extraction and the operation method of PCR reaction have not changed, but a simple liquid is made between the two Transfer, this method seems simple, but there are many hidden dangers. It is easy to produce aerosol pollution when multiple samples are processed at the same time. When doing a single sample, the entire system structure is even larger and more complicated.

Images