A kind of purification method of long-chain dibasic acid

A long-chain dibasic acid and purification method technology, applied in the field of biochemical industry, can solve the problems of product color and nitrogen content, limited removal effect, etc., and achieve high product yield, improved removal rate, and environmentally friendly effects.

Active Publication Date: 2021-02-05
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for water-soluble proteins and other impurities in the fermentation broth, only filtration and repeated acidification and dilution are used, and the removal effect is limited. Heating the system to high temperature before crystallization will also promote the oxidation and denaturation of pigments and proteins. The reduction will be adsorbed in the crystal product, which will have a certain impact on the color and nitrogen content of the product

Method used

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  • A kind of purification method of long-chain dibasic acid

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Embodiment 1

[0033] Using n-dodecane as substrate, use Candida tropicalis to ferment and produce dodecane dibasic acid. At the end of the fermentation, the concentration of dodecanedibasic acid was 150g / L, and the pH was 7.0. Take 1000ml of fermentation broth, heat it to 90°C, let it stand to room temperature, separate the residual alkanes in the upper layer, filter to remove the bacteria, and obtain the fermentation supernatant. While stirring, slowly add 6 mol / L sulfuric acid to the fermentation clear liquid to adjust the pH to 5.0, continue stirring for 30 minutes, then add 10 mol / L sodium hydroxide solution to the fermentation clear liquid while stirring, adjust the pH to 8.0, and continue stirring for 30 minutes , and then add 5g of sodium lauryl sulfate to the clear liquid, and continue to stir for 60min. Use a membrane with a pore size of 10 -2 μm membrane filter to remove the precipitate. Add 5 g of activated carbon to the filtered clear liquid, stir and decolorize for 20 min, a...

Embodiment 2

[0035] Tridecane dibasic acid was produced by fermenting Candida tropicalis with n-tridecane as substrate. At the end of the fermentation, the tridecanedioic acid concentration was 145g / L, and the pH was 7.2. Take 1000ml of fermentation broth, heat it to 95°C, let it stand to room temperature, separate the residual alkanes in the upper layer, filter to remove the bacteria, and obtain the fermentation supernatant. While stirring, slowly add 8 mol / L nitric acid to the fermentation clear liquid to adjust the pH to 4.0, continue stirring for 20 minutes, then add 10 mol / L potassium hydroxide solution to the fermentation clear liquid under stirring, adjust the pH to 9.0, and continue stirring for 20 minutes , and then add 15g isomeric tridecanol polyoxyethylene ether to the clear liquid, and continue to stir for 3h. Use a membrane with a pore size of 10 -2 μm membrane filter to remove the precipitate. Add 10 g of activated carbon to the filtered clear liquid, stir and decolorize ...

Embodiment 3

[0037] Tetradecane dibasic acid was produced by fermenting Candida tropicalis with n-tetradecane as substrate. At the end of the fermentation, the tetradecanedibasic acid concentration was 135g / L, and the pH was 7.4. Take 1000ml of fermentation broth, heat it to 95°C, let it stand to room temperature, separate the residual alkanes in the upper layer, filter to remove the bacteria, and obtain the fermentation supernatant. While stirring, slowly add 4 mol / L hydrochloric acid to the fermentation clear liquid to adjust the pH to 4.5, continue stirring for 40 minutes, then add 6 mol / L sodium hydroxide solution to the fermentation clear liquid while stirring, adjust the pH to 9.5, and continue stirring for 40 minutes , and then add 20g of sodium dodecylsulfonate to the clear liquid, and continue to stir for 2h. Use a membrane with a pore size of 10 -2 μm membrane filter to remove the precipitate. Add 3g of activated carbon to the filtered clear liquid, stir and decolorize for 40m...

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Abstract

The invention relates to a purification method of a long-chain dicarboxylic acid, which comprises I, heating a terminated fermenting liquid for demulsification, filtering to obtain a fermenting clearliquid; II, stirring while adjusting pH of the fermenting clear liquid to acid level, adjusting pH to basic level, adding little surfactant, stirring for a while, and filtering to remove solid matters; III, adding activated carbon, stirring, decoloring, and filtering; IV, heating the clear liquid of step III for acidifying, cooling, filtering, and drying to obtain the finished long-chain dicarboxylic acid. Synergy of an acid, a base and a surfactant herein helps effectively reduce the nitrogen content in the bio-process long-chain dicarboxylic acid, so that a high-quality dicarboxylic acid isattained.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a method for purifying long-chain dibasic acids, in particular to a method for obtaining high-purity long-chain dibasic acids from microbial fermentation liquid. Background technique [0002] The molecular formula of long-chain dibasic acid is C n h 2n-2 o 4 , where n is 10-18, which is a metabolite obtained by fermentation of microorganisms using liquid wax, etc. Its fermentation broth is a complex multiphase system, which contains unreacted carbon sources, microbial cells and debris, unused medium and metabolites, and microbial secretions, etc., especially containing a large amount of impurities such as proteins, pigments, and ash , have a strong impact on the purity and application of the product, and bring difficulties to the extraction and purification of this type of dibasic acid. [0003] At present, the methods for extracting long-chain dibasi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C51/42C07C51/487C07C51/43C07C55/02C07C55/21C11C1/02C11C1/08
CPCC07C51/42C07C51/43C07C51/487C11C1/02C11C1/08C07C55/02C07C55/21
Inventor 李晓姝高大成张霖师文静樊亚超王鹏翔孙启梅
Owner CHINA PETROLEUM & CHEM CORP
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