Multichannel tumor marker parallel detection sensor and use method thereof

Abstract

A multifunctional tumor marker parallel detection sensor and a use method thereof relate to the field of micro-nano medical detection application technology. A plurality of nano-hole sandwich array chips are supplied as a substrate. One side, which is next to a waste liquid outlet, of each liquid pool of a base is provided with a plurality of flow channels. Each flow channel is communicated with aliquid pool. Each liquid pool of the base is provided with a chip. Each liquid pool is bonded with a PDMS cover board. The nano-hole sandwich array chips are respectively placed at the central part of each liquid pool of the base flatly. Penetration of liquid at the upper side and the lower side of each liquid pool can only be realized through the nano-hole array. Then the inner surface of the middle-layer nano-hole of the substrate of each channel is modified by tumor marker antibody modules. Then a testing sample is conveyed to a testing area by a precise injection pump through each channel. Parallel detection for a plurality of tumor markers is performed. Tumor marker modules are captured by the antibody modules in the nano-hole sandwich array chip, and furthermore blocking current isgenerated. Concentration determining of the tumor marker is realized according to the reduction amplitude of the blocking current.

Description

technical field [0001] The invention relates to the technical field of biosensors, in particular to a multi-channel tumor marker parallel detection sensor and its application method. Background technique [0002] Cancer is the second leading cause of human death, and new cancer cases in my country account for about 1 / 4 of the world's total. The World Health Organization points out that 1 / 3 of cancers can be cured in time through early diagnosis. Tumor markers are substances produced directly by tumor cells or induced by non-tumor cells through tumor cells. Studies have shown that when tumors develop, the concentration of markers is significantly abnormal, indicating the presence of tumors. Tumor marker detection research provides new ideas for non-invasive early diagnosis of cancer. Quantitative detection of tumor markers using micro-nano sensors with special effects such as Coulomb blockade and quantum size effects has also become a major research method for early diagno...

AI Technical Summary

Problems solved by technology

[0004] Studies have shown that the time for a single tumor marker to pass through the nanopore is at the millisecond level, and the time for femtomole-level quantitative detection is more than 100 hours, and the detection efficiency is low

At the same time, there are many forms of single molecules passing through the nanopore, which will generate a variety of blocking current signals and reduce the detection accuracy.

In addition, the tumor markers of a single cancer have diverse characteristics, so realizing the parallel detection of multi-channel tumor markers is a severe challenge for early detection of cancer

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