Application of circ-FAM193A molecular marker in blood in diagnosis and prognosis of esophageal squamous carcinoma

A circ-fam193a, 1.circ-fam193a technology, applied in the determination/examination of microorganisms, biochemical equipment and methods, etc., can solve the problems of unacceptable patients and large trauma

Active Publication Date: 2019-04-05
江苏万成生物医学研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in esophageal squamous cell carcinoma, circRNAs are rarely reported
At present, ESCC does not have plasma tumor markers to evaluate the condition. Endoscopy is the gold standard, but it is traumatic and difficult for patients to accept.

Method used

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  • Application of circ-FAM193A molecular marker in blood in diagnosis and prognosis of esophageal squamous carcinoma
  • Application of circ-FAM193A molecular marker in blood in diagnosis and prognosis of esophageal squamous carcinoma
  • Application of circ-FAM193A molecular marker in blood in diagnosis and prognosis of esophageal squamous carcinoma

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Embodiment 1

[0033] 1. RNase R Digestion and Actinomycin-D Digestion

[0034] 2mg of total RNA was incubated at 37°C for 20 minutes with or without the addition of 3U / mg RNase R, followed by the use of the RNeasyMinElute kit. For actinomycin D treatment, 2 mg of total RNA was incubated at 37°C with or without adding 1 mg of actinomycin (actinomycin D, purchased from Sigma Reagent Company), and at 0, 6, 12, The resulting RNA was detected separately for 18 hours.

[0035] 2. Subjects

[0036] This study is based on the Department of Oncology, Nanjing Hospital Affiliated to Nanjing Medical University. The Department of Oncology treats patients with esophageal squamous cell carcinoma all the year round, and establishes a specimen bank, which can provide rich case data for this study. The tissues and blood of the study patients came from patients with esophageal squamous cell carcinoma who underwent radical or palliative surgical resection from 2013 to August 2017. The normal control blood s...

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Abstract

The invention belongs to the field of oncomolecularbiology and specifically relates to application of a circ-FAM193A molecular marker in blood in diagnosis and prognosis of esophageal squamous carcinoma. A detection reagent for circ-FAM193A molecular marker can be used for preparing a diagnostic reagent for esophageal squamous carcinoma; circular RNA has the characteristics of stable organization,high abundance and sample specific expression; expression quantity of circ-FAM193A in blood samples of an ESCC patient and a normal person has a difference and is associated with the stage; the method can be easily accepted by subjects and can become an effective method for early detection and judgement for disease progression of the ESCC patient.

Description

technical field [0001] The invention belongs to the field of tumor molecular biology, and specifically relates to the application of circ-FAM193A molecular marker in blood in the diagnosis and prognosis of esophageal squamous cell carcinoma. Background technique [0002] Esophageal cancer is one of the most common malignant tumors that seriously threaten human health. There are two main pathological types: squamous cell carcinoma and adenocarcinoma. Ninety percent of esophageal cancers are squamous cell carcinomas (ESCC), and their distribution has obvious geographical differences. The incidence of ESCC in my country accounts for more than 70% of the world, and the prognosis is poor. The 5-year overall survival rate is only 15%-25%. In 2016, the Journal of Clinician Cancer reported that esophageal cancer has become one of the five most common tumors in China, ranking third among men and fifth among women. [0003] Circular RNAs (circRNAs), a new type of endogenous non-codi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/178C12Q2600/118
Inventor 康宁朱桂芳曹秀峰
Owner 江苏万成生物医学研究院有限公司
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