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Seed tissue-culturing propagation method of iris

An iris group and seed technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of restricting the progress of iris tissue culture expansion, low ramet reproduction coefficient, and high pollution rate

Pending Publication Date: 2019-04-12
余小芳
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Problems solved by technology

[0003] Most of the domestic iris seedlings are imported from abroad, the number is small, the hybridization rate between iris varieties is low, and the seeds are generally dormant, the dormancy period is long, the emergence rate is low, and the ramet reproduction coefficient is low, and it takes a long time for seedling propagation The explants of iris tissue culture mostly use rhizomes, but the rhizomes are in contact with soil, there are many endophytes, the pollution rate is high, and there are few basic materials, it is difficult to obtain a large number of explants in a short period of time, which greatly limits the expansion of iris tissue culture. complicated progress
Therefore, the process of iris breeding has been greatly affected

Method used

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Embodiment Construction

[0015] The present invention will be further described below. It should be noted that the following examples are based on the technical solution and provide detailed implementation and specific operation process, but the protection scope of the present invention is not limited to this example.

[0016] A method for tissue culture propagation of iris seeds, comprising the following steps.

[0017] S1 Put the seeds into a beaker with concentrated sulfuric acid and soak for 5 minutes, slowly pour the concentrated sulfuric acid into water to dilute for about 5 minutes, and take out the iris seeds after the dilution is completed as soon as possible.

[0018] S2 Rinse the soaked seeds with running water for 30 minutes and bring them into the aseptic operating table; soak them in 75% alcohol and stir them for 5 minutes, then rinse them with sterile water for 3 to 5 times; then soak them in 5% sodium hypochlorite solution and stir them After 3 minutes, rinse with sterile water 5-6 tim...

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Abstract

The invention discloses a convenient method for directly culturing iris seed as root seedlings, and relates to the field of the plant tissue culture and seed dormancy breaking. The method comprises the following steps: taking mature seeds of the iris as objects, breaking the seed dormancy by using a chemical measure, and directly forming seedlings and rooting through a culture medium. The breedingprocess is greatly shortened through the above way, and the cost is reduced. The most proper improved culture medium is screened so that the culture medium meets a breeding target. By adopting the seed to perform tissue-culture, the obtained seedling is low in pollution rate and high in differentiation rate; the genetic shape is stable, and the effective substance content is high; the operation is simpler and easy to operate in comparison with the root stock, anther and like explants.

Description

technical field [0001] The invention belongs to the technical field of iris seedling cultivation, and in particular relates to a method for tissue culture propagation of iris seeds. Background technique [0002] Iris (Iris), the perennial root flower of the Iridaceae (Iridaceae), Iris plants have peculiar flower postures, gorgeous and rich colors, various varieties, strong adaptability, and are widely used in gardens. They are greening, beautifying and fragrant cities, and decorating flower beds. , flower paths, flower belts, roadsides and lawns are excellent materials. Its rhizome can be used to extract essence and also be used as traditional Chinese medicine. Multi-use division, sowing method propagation. [0003] Most domestic iris seedlings are imported from abroad, the number is small, the hybridization rate between iris varieties is low, and the seeds are generally dormant, the dormancy period is long, the emergence rate is low, and the ramet reproduction coefficient...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01C1/02
CPCA01C1/02A01H4/001A01H4/008
Inventor 余小芳赵梓安周永红
Owner 余小芳
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