Group a rotavirus test strips

A technology of rotavirus and immunochromatographic test paper, which is applied in the field of immunological detection, can solve the problems of affecting the sensitivity of detection results, the detachment of antigen and antibody, and the reduction of antigen and antibody, and achieve the effect of improving anti-interference ability and detection sensitivity

Active Publication Date: 2022-02-08
SICHUAN MACCURA BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, after the colloidal gold particles are connected to the antigen and antibody, they are very unstable, and it is easy to detach the antigen and antibody due to the change of charge, resulting in a greatly reduced amount of antigen and antibody connected to each colloidal gold particle, resulting in a decrease in sensitivity.
[0008] Therefore, the environmental pH, ionic strength, the amount of colloidal gold particles, the molecular weight of the antigen and antibody, and the concentration of the antigen and antibody will all affect the adsorption of the antigen and antibody, thereby affecting the sensitivity of the test results. Therefore, although the immune colloidal gold technology is a comparative Mature method, but the detection effect is still different for each specific detection substance
[0009] On the other hand, when detecting rotavirus antigens in stool samples based on the immunocolloidal gold method, there are also problems of interference from various substances such as food residues, drug residues, hemoglobin, and white blood cells in the stool samples.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Labeling of the first anti-group A rotavirus monoclonal antibody

[0063] Slowly add an appropriate amount of labeling buffer into the colloidal gold solution and mix well, then add an appropriate amount of the first anti-group A rotavirus monoclonal antibody, and finally add the stop solution to form the first anti-group A rotavirus monoclonal antibody- The colloidal gold complex is then moderately centrifuged to obtain a purified colloidal gold-labeled protein complex, which is sprayed onto the colloidal gold pad.

Embodiment 2

[0064] Example 2 Coating of the second anti-group A rotavirus monoclonal antibody

[0065] Put the membrane coating solution on the operating table, first accurately measure the membrane coating solution and add it to the corresponding clean container, then measure the second anti-group A rotavirus monoclonal antibody and add it to the corresponding membrane coating solution, mix well, Make the final concentration of the second anti-group A rotavirus monoclonal antibody at the detection line (T line) be 1.2 mg / mL.

Embodiment 3

[0066] Coating of embodiment 3 anti-animal IgG antibodies

[0067] Put the membrane coating solution on the operating table, first accurately measure the required membrane coating solution and add it to the corresponding clean container, then measure the required goat anti-mouse IgG polyclonal antibody and add it to the corresponding membrane coating solution, mix well, Make the final concentration of the goat anti-mouse IgG polyclonal antibody at the quality control line (C line) be 1 mg / mL.

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Abstract

The invention relates to a colloidal gold immunochromatographic test paper for detecting group A rotavirus. The colloidal gold immunochromatographic test paper includes a chromatographic membrane, and the chromatographic membrane is provided with a detection line and a quality control line; wherein , the colloidal gold is coated with a first anti-group A rotavirus monoclonal antibody, and the detection line is coated with a second anti-group A rotavirus monoclonal antibody. The invention also relates to corresponding reagents. Improve anti-interference ability and sensitivity when detecting group A rotavirus.

Description

technical field [0001] The invention relates to the field of immune detection, in particular to a colloidal gold immunochromatographic test paper for detecting group A rotavirus. Background technique [0002] Rotavirus (RV) is a double-stranded RNA virus belonging to the genus Rotavirus of the family Reoviridae, and is the main pathogen that causes non-bacterial diarrhea in infants and various young animals. It mainly infects the epithelial cells of the small intestine, causing cell damage and causing diarrhea. Rotavirus is prevalent in summer, autumn and winter every year. The route of infection is the fecal-oral route. The clinical manifestations are acute gastroenteritis and osmotic diarrhea. Days, severe dehydration symptoms. [0003] There are three kinds of antigens on the surface of RV particles, namely group antigen (VP6), neutralizing antigen (VP7) and hemagglutinin antigen (VP4). The group antigen is related to a variety of structural proteins, mainly the inner ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569G01N33/558
CPCG01N33/558G01N33/56983G01N2333/14
Inventor 杨丹易小钧刁维均
Owner SICHUAN MACCURA BIOTECH CO LTD
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