A detection method for the survival status of chicken embryos based on the frequency characteristics of absorption spectrum

Abstract

The invention relates to a method for detecting the living state of chicken embryos based on the frequency characteristics of absorption spectra, which comprises the following steps: using a light source to irradiate eggs containing chicken embryos, acquiring absorption spectrum information and performing normalization processing and recording it as x(n); Perform second-order wavelet transform on x(n), take its second-order detail signal and record it as d(n); perform Fourier transform on d(n) and record it as D(k), k=1,2..., N, N is the number of sequences; in D(k), calculate the ratio R of the spectrum energy in the specific frequency band of the chicken embryo to the total energy in the whole frequency band; find the maximum value P of the spectral peak in the specific frequency band; according to the prior logic rules The survival status of chicken embryos was determined according to the decision tree model.

Description

technical field [0001] The invention provides a non-invasive detection method for the living state of chicken embryos in eggs based on absorption spectrum frequency characteristics, which belongs to a production-oriented spectral signal processing application. Background technique [0002] At present, domestic bird flu vaccines, animal flu vaccines such as swine flu, canine flu, and some human vaccines such as influenza A and hepatitis B vaccines are mostly produced using the "chicken embryo method", that is, the method of cultivating viruses in embryonated eggs. Chicken embryos are the carrier of virus culture. Chicken embryos are strictly screened, considering whether they are fertilized, whether they are polluted, the number of days, and their size. After a period of cultivation, the virus is extracted from chicken embryos, and after inactivation and other processes, the vaccination vaccine is produced. [0003] The survival of chicken embryos and eggs is closely related...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of existing manual detection methods for the viability of chicken embryos in eggs in terms of detection efficiency, detection accuracy, and detection speed, the present invention proposes a detection method for the survival status of chicken embryos in eggs based on specific spectral band absorption spectrum frequency characteristics. The non-destructive and fast optical detection method can determine the survival status of chicken embryos in a simple and feasible way. Compared with manual judgment and other semi-automatic judgment methods, it has the characteristics of non-invasive, non-contact and rapid detection, and the detection speed and accuracy have been significantly improved.

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