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Low temperature diluent of pig sperm based on Pelodiscus sinensis hibernating serum

A technology of soft-shelled soft-shelled turtle and diluent, which is applied in the field of sperm diluent, can solve the problems of high sperm energy consumption, affecting the vitality and survival rate of pig sperm, and achieve the effect of good integrity

Inactive Publication Date: 2019-04-19
ZHEJIANG WANLI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the in vitro storage of pig sperm is usually stored at 16.5-17°C. Once the temperature is lower than this temperature, the vitality and survival rate of pig sperm will be seriously affected.
Pig sperm is very sensitive to temperature changes, especially cooling, but high temperature will lead to high energy consumption of sperm, so it is necessary to study the dilution of pig sperm cryopreservation

Method used

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  • Low temperature diluent of pig sperm based on Pelodiscus sinensis hibernating serum
  • Low temperature diluent of pig sperm based on Pelodiscus sinensis hibernating serum

Examples

Experimental program
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Effect test

Embodiment 1

[0017] This embodiment provides a low-temperature dilution of pig sperm based on the Chinese soft-shelled turtle hibernation serum. The low-temperature dilution contains 40 mL of Chinese soft-shelled turtle hibernation serum, 20 mL of centrifuged egg yolk liquid, and 30.0 g of fructose per 1 L of the dilution. , 40.0 g of sucrose, 4.0 g of sodium citrate, 0.6 g of penicillin sodium, and 0.6 g of streptomycin. The preparation method is to dissolve the above-mentioned raw materials in the aqueous solution after being stirred, filter and sterilize, and freeze for later use; when used, it can be isothermally diluted with sperm according to a certain volume ratio.

[0018] The preparation method of the Chinese soft-shelled turtle hibernating serum is to draw blood to obtain the Chinese soft-shelled turtle hibernating serum after the Chinese soft-shelled turtle hibernates, freeze it at ultra-low temperature, and wait for use; After removing the precipitate, keep the supernatant and ...

Embodiment 2

[0022] A low-temperature dilution of pig sperm based on Chinese soft-shelled turtle hibernation serum provided in this example differs from Example 1 in that the low-temperature dilution contains 30 mL of Chinese soft-shelled turtle hibernation serum per 1 L of dilution, and is centrifuged for later use. yolk liquid 15mL, fructose 25g, sucrose 30g, sodium citrate 3g, penicillin 0.5g, streptomycin 0.5g.

Embodiment 3

[0024] A low-temperature dilution of pig sperm based on the Chinese soft-shelled turtle hibernation serum provided in this example differs from Example 1 in that the low-temperature dilution contains 50 mL of Chinese soft-shelled turtle hibernation serum per 1 L of the dilution, and is centrifuged for later use. yolk liquid 25mL, fructose 35g, sucrose 50g, sodium citrate 5g, penicillin 0.8g, streptomycin 0.8g.

[0025] After testing, pig sperm preserved for 5 days without the diluent of Chinese soft-shelled turtle hibernation serum had poor acrosome integrity; pig sperm preserved with dilute solution of Chinese soft-shelled turtle hibernation serum for 5 days had good acrosome integrity. Test results such as Figure 1-2 As shown, the test data are shown in Table 1.

[0026] Table 1 The effect of the low temperature dilution of pig sperm based on the hibernation serum of soft-shelled turtle of the present invention on sperm motility

[0027] temperature

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Abstract

The invention discloses a low temperature diluent of pig sperm based on Pelodiscus sinensis hibernating serum, which includes hibernating serum of Pelodiscus sinensis, yolk liquid of Pelodiscus sinensis, buffering salts, nutritional components and antibiotics. The low temperature diluent of pig sperm based on Pelodiscus sinensis hibernating serum of the invention is added by the hibernating serumof Pelodiscus sinensis so as to reduce the in vitro storage temperature of the pig sperm to 4 degrees Celsius, with the most suitable temperature being 6 to 8 degrees Celsius. Histological analysis ofcryopreserved sperm shows that the integrity of sperm acrosome is obviously better than that of normal temperature preservation.

Description

technical field [0001] The invention relates to a sperm diluent, in particular to a pig sperm low-temperature diluent based on the Chinese soft-shelled turtle hibernation serum. Background technique [0002] With the continuous intensification, specialization, ecologicalization and technological development of my country's pig industry, the artificial insemination technology of pigs has received more and more attention in the modern pig industry. However, the in vitro storage of pig sperm is usually stored at 16.5-17°C. Once the temperature is lower than this temperature, the vitality and survival rate of pig sperm will be seriously affected. Pig sperm is very sensitive to temperature changes, especially cooling, but higher temperature will lead to high energy consumption of sperm, so it is necessary to study the dilution of pig sperm for cryopreservation. Contents of the invention [0003] The purpose of the present invention is to provide a low-temperature dilution of p...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0215A01N1/0221A01N1/0226
Inventor 陈忠法王伟钱国英尹尚军
Owner ZHEJIANG WANLI UNIV
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