Ilex verticillata pollen preservation method

Abstract

The invention relates to an ilex verticillata pollen preservation method. The ilex verticillata pollen preservation method comprises the following steps: S1, collecting a pollen: collecting the ilex verticillata pollen; choosing to collect the pollen of a staminiferous plant, wherein the staminiferous plant grows robustly and has no obvious diseases, the bud blooms, the anther can be seen, and more than 30% of the anther becomes yellow; step 2, drying the pollen: drying the collected ilex verticillata pollen; step 3, refrigerating the pollen: putting the dried ilex verticillata pollen into a freezing pipe, wrapping a thin gauze on the surface of the freezing pipe, placing a small quantity of weight into the gauze, wherein the weight can be small iron nails, then the freezing pipe can be immersed in liquid nitrogen after being put into the liquid nitrogen, tying the gauze with a thin rope, then directly putting the gauze into the liquid nitrogen, and storing the pollen at the ultralow temperature of minus 170 DEG C through the liquid nitrogen; the ilex verticillata pollen preservation method guarantees the vitality of the ilex verticillata pollen, guarantees the germination rate ofthe pollen, and has broad popularization and application values.

Description

technical field [0001] The invention belongs to the technical field of plant high-yield cultivation, and in particular relates to a method for preserving North American holly pollen. Background technique [0002] North American holly (Ilex verticillata) is an excellent tree species for cutting branches and ornamental fruit and potted Chinese New Year flowers. Another notable feature of it is dioeciousness, which is a typical insect-borne flower of an excellent nectar source plant. In its cultivation, it was found that the pollination conditions of North American holly are very harsh, and in nature it is very dependent on bees to help complete pollination. However, if the flowering period of North American holly encounters the rainy season or the female flowers cultivated in greenhouses are in full bloom, the bees may be due to the rainy season. The influence of high temperature does not work, and at this time North American holly just cannot complete pollination under natur...

AI Technical Summary

Problems solved by technology

In the production and cultivation in recent years, it has also been found that the male plants of North American holly have poor resistance and are prone to poor growth, causing the female plants to be short or dead, and it is even more difficult to meet the pollination needs of the female plants in the plantation garden.

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