Integrated automatic pretreatment device for high-throughput chromatographic detection

Abstract

The invention discloses an integrated automatic pretreatment device for high-throughput chromatographic detection, and aims to integrate chemical reaction, uniform mixing, centrifugation, incubation and sample introduction involved in dairy product pretreatment into an automatic device, improve the pretreatment efficiency of to-be-detected dairy products and realize one-stop pretreatment. The device comprises an integrated centrifuge, a plunger pump, a three-way valve and a multi-channel incubation-sample introduction device. The plunger pump is used for providing power for the whole pretreatment device, so as to realize drawing a treated target solution in the integrated centrifuge, filling of an incubation solution in the multi-channel incubation-sample introduction device and synchronous sample introduction after incubation are completed; the three-way valve provides a path for conveying liquid; the integrated automatic pretreatment device for high-throughput chromatographic detection provided by the invention can be used for treating materials such as dairy products, blood and tissues; the device can be used for sample pretreatment in the fields of clinical diagnosis, inspection and quarantine, food safety detection, judicial identification, drug detection and the like, and has a wide market prospect.

Description

technical field [0001] The invention relates to a chemical reaction device, in particular to an integrated automatic pretreatment device for high-throughput chromatography detection, belonging to the technical field of biomedicine. Background technique [0002] Chromatography is the abbreviation of "chromatographic analysis". Taking the commonly used immunochromatography as an example, the principle is to fix the specific antibody to a specific zone of the nitrocellulose membrane, and then add the sample dropwise to the membrane. Under the action of the capillary, the sample moves forward along the membrane, and when it moves to the area where the antibody is immobilized, the corresponding antigen in the sample will specifically bind to the antibody. At this time, if immunological nanoparticles such as colloidal gold or immunozyme are used for staining, or combined with marker molecules with Raman, chemiluminescence, upconversion luminescence, and time-resolved fluorescence ...

AI Technical Summary

Problems solved by technology

Taking the device for detecting chemical pollution indicators in dairy products (test strips, test cards) as an example, its disadvantages are: (1) Most of the detection indicators are directly measured on dairy products without pretreatment of milk samples. Simple and fast signal response, the biggest disadvantage is that the complex matrix environment of milk samples significantly restricts the detection sensitivity; (2) the inconsistency of the pre-processing methods of the detection indicators makes it difficult to achieve high-throughput determination of the dairy products to be tested

At present, the only high-throughput devices on the market are mostly high-throughput signal readers; (3) For the detection indicators that require pre-incubation, multi-channel simultaneous incubation and measurement before detection have not been realized, which significantly restricts the detection throughput.

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