DELFIA-based pesticide residue detection device and method

Abstract

The invention provides a DELFIA-based pesticide residue detection device and method. The device comprises an analyzer having a centrifugal disk and a fluorescence detecting mechanism, and a microfluidic chip fixedly arranged on the centrifugal disk and acquiring a detection liquid by a centrifugal effect, wherein a sample hole, a filter hole, a first reaction hole, a second reaction hole and a waste liquid hole are successively arranged in the microfluidic chip from the inside to the outside in the radial direction of the centrifugal disk; the sample hole, the filter hole, the first reaction hole, the second reaction hole and the waste liquid hole communicate successively through micro-channels; sugar membranes of different thicknesses are disposed at the openings of the micro-channels, and open the micro-channels according to different centrifugal rotation speeds; the sample hole is used for containing a sample solution of a pesticide residue detection liquid, and the sample solutionis evenly stirred by centrifugal rotation. Thus, the harmful component content in a crop sample is detected. The device has few manual operation processes, is easy to operate, and can rapidly detect the pesticide residue sample solution.

Description

technical field [0001] The invention relates to the field of pesticide residue inspection, in particular to a DELFIA-based pesticide residue detection device and detection method. Background technique [0002] The development of agricultural industrialization makes the production of agricultural products more and more dependent on exogenous substances such as pesticides, antibiotics and hormones. The amount of pesticides used in agricultural products in my country remains high, and the unreasonable use of these substances will inevitably lead to excessive pesticide residues in agricultural products, affecting the safety of consumers, and in severe cases, it will cause consumers to become ill, develop abnormally, and even directly lead to poisoning die. Excessive pesticide residues will also affect the trade of agricultural products. Countries all over the world attach great importance to the issue of pesticide residues, and have set increasingly strict limits for pesticide r...

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Problems solved by technology

[0004] The above method needs to add reagents in each step of operation and then operate according to the standard, resulting in the use of more reagents and many steps, and the operation is complicated

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