Cervical cancer biomarker and test kit thereof

A technology of biomarkers and detection kits, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of reducing the mortality rate of cervical cancer patients, low diagnostic sensitivity and specificity, and high false-negative rate, so as to improve the diagnostic rate. , Reduced inter-individual differences, high specificity effect

Active Publication Date: 2019-06-25
天津云检医学检验所有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0002] Cervical cancer is a major health problem for women in the world. In 2012, the global incidence of cervical cancer was 528,000, and the annual death rate was 266,000. It is the most common cancer among women in the world, and 85% of cases occur in developing countries. Cervical cancer is The main cause of cancer death in women, the prognosis of patients with cervical cancer is closely related to the clinical stage, most patients with early stage cervical cancer can be successfully cured, but the survival rate of patients with advanced cervical cancer is only 15%, therefore, early diagnosis and treatment can Significantly reduce the mortality rate of cervical cancer patients, cervical

Method used

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  • Cervical cancer biomarker and test kit thereof
  • Cervical cancer biomarker and test kit thereof
  • Cervical cancer biomarker and test kit thereof

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Experimental program
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Effect test

Embodiment 1

[0043] A detection kit for cervical cancer biomarkers, including one or more biomarker capture reagents corresponding to the above biomarkers, the specification is 5ml (3ug / ml), polystyrene solid phase carrier, AlexaFluor680 fluorescent dye reagent, when using, take a certain amount of antibody reagent, put the solid phase carrier into the antibody reagent, make the antibody adsorb on the solid phase carrier, put the solid phase carrier and fluorescent dye reagent into the reagent to be detected at the same time After reacting for a period of time, it was detected by a fluorescence spectrophotometer.

Embodiment 2

[0045] A detection kit for cervical cancer biomarkers, including one or more biomarker capture reagents corresponding to the above biomarkers, the specification is 5ml (3ug / ml), solid phase carrier: iPDMS film, chemical Luminescent material: oxalyl chloride, solubilizing buffer: PBS solution, cleaning agent: Tween 20.

[0046] The present invention uses the marker protein screened out from biological samples as a novel cervical cancer biomarker, which has the advantages of convenient material collection, low detection cost, good sensitivity and high specificity, and is widely used in the preparation, prediction, diagnosis, identification or evaluation of cervical cancer The reagents have become an effective means for early screening of cervical cancer. In addition, through a variety of cervical cancer biomarkers, it can effectively overcome the defects of low specificity and low sensitivity caused by differences between individuals, and significantly improve the diagnosis rate ...

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Abstract

The invention provides a cervical cancer biomarker. The cervical cancer biomarker comprises one or more of marker proteins stably present in a biological sample: DEPDC1, POLQ, MCM10, ASPM, TPX2, JPH4,CDC45, MYBL2, PAGE4, NCAPG, FOXM1, KIF20A, CDC20, NEK2, CASQ2, PRLHR, SLITRK3, HJURP, DLGAP5, TOP2A, MYH11, TROAP, SCN7A, CEP55, NUF2, CLVS2, MKI67, GPM6A, BUB1B, CD300LG, LMOD1, TCF23, CLEC3B, MELK, RRM2, EXO1, KIF18B, ADH1B, PEG3, TCEAL6, PGM5-AS1, ADAMTS9-AS2, CARMN, RP11-887P2.5, FR D6-AS2, FXYD1, LCN6, UHRF1, GPIHBP1 and AC053503.12. According to the invention, the marker protein selectedfrom the biological sample is used as a novel cervical cancer biomarker, which has the advantages of convenient material selection, low detection cost, good sensitivity and high specificity; through avariety of cervical cancer biomarkers, the defects of low specificity and low sensitivity caused by differences between individuals can be effectively overcome; and the diagnosis rate of a disease issignificantly improved. The invention further provides a cervical cancer biomarker test kit.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular to a cervical cancer biomarker and a detection kit. Background technique [0002] Cervical cancer is a major health problem for women in the world. In 2012, the global incidence of cervical cancer was 528,000, and the annual death rate was 266,000. It is the most common cancer among women in the world, and 85% of cases occur in developing countries. Cervical cancer is The main cause of cancer death in women, the prognosis of patients with cervical cancer is closely related to the clinical stage, most patients with early stage cervical cancer can be successfully cured, but the survival rate of patients with advanced cervical cancer is only 15%, therefore, early diagnosis and treatment can Significantly reduce the mortality rate of cervical cancer patients, cervical smear cytology is the main screening method for early diagnosis of cervical cancer, but the shortcomings of...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/533
Inventor 凌雪峰
Owner 天津云检医学检验所有限公司
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