DNA-carrier-based Chinese character information storing method

Abstract

The invention provides a DNA-carrier-based Chinese character information storing method. The method comprises the following processes that S1, text and punctuation marks are coded with ciphers takingfour bases as a group, and then the text is generated into a coding information table; S2, according to coding information, the text is converted into a nucleic acid sequence, in the nucleotide sequence, the single nucleotide repeat is not more than 6 bp, and the GC content is between 45% and 60%; S3, 20 bp of nucleotide sequence used for preventing a stored information signal from losing in the sequencing process is added at the two ends of a stored information interval, the sequence is synthesized randomly, and it is only guaranteed that the single nucleotide repeat is not more than 3 bp; S4, a primer binding interval is located at the upstream and the downstream of the stored information interval, a selected PRC primer binding interval is located at the upstream and the downstream of the stored information interval, and selected PRC primers are a positive primer 5'-TACGCTATGAACCACTTA-3' and a reverse primers 5'-TAACATCAGACCTCTTCGCA-3'; S5, nucleotide fragments are synthesized sequentially according to the designed nucleotide sequences, and the artificially synthesized coded information DNA sequence is linked to plasmid and then transformed into bacteria.

Description

technical field [0001] The invention relates to the technical field of DNA storage, in particular to a method for storing Chinese character information based on a DNA carrier. Background technique [0002] With the continuous progress of society, the data information generated by human beings is increasing exponentially. Currently, the more popular data storage media mainly include CDs, tapes, hard disks, U disks, SM cards, memory sticks, etc. Although these storage media are constantly being upgraded, their storage capacity is still difficult to meet the exponential growth of big data. At the same time, these storage media have a short service life and can be used for decades at most. After decades, the permanently stored data needs to be transferred to In the transfer process, due to the large data capacity, the data is easily lost. At the same time, continuous maintenance and maintenance of storage devices is required, which costs a lot of money. Discarded storage devic...

AI Technical Summary

Problems solved by technology

Although the high cost of DNA synthesis and sequencing limits the widespread application of DNA storage technology, the price of DNA synthesis and sequencing has dropped exponentially, far faster than the price of traditional storage devices.

Images