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Combined preparation of umbilical cord mesenchymal stem cells and exosomes for treatment of erectile dysfunction, and preparing method and drug administration method thereof

A technology of erectile dysfunction and mesenchymal stem cells, which is applied in the field of joint preparations of umbilical cord mesenchymal stem cells and exosomes, can solve the problems that cells are difficult to reach the penis and the treatment effect is limited, so as to maintain activity and function and improve penis blood supply, improve the effect of treatment

Inactive Publication Date: 2019-07-02
天津欣普赛尔生物医药科技有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

However, direct intravenous reinfusion of umbilical cord mesenchymal stem cells has limited therapeutic effect because the cells are difficult to reach the penis

Method used

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  • Combined preparation of umbilical cord mesenchymal stem cells and exosomes for treatment of erectile dysfunction, and preparing method and drug administration method thereof
  • Combined preparation of umbilical cord mesenchymal stem cells and exosomes for treatment of erectile dysfunction, and preparing method and drug administration method thereof
  • Combined preparation of umbilical cord mesenchymal stem cells and exosomes for treatment of erectile dysfunction, and preparing method and drug administration method thereof

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Experimental program
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preparation example Construction

[0019] The preparation method of the combined preparation of umbilical cord mesenchymal stem cells and exosomes for treating erectile dysfunction of the present invention comprises the following steps:

[0020] (1) Cultivation of human umbilical cord mesenchymal stem cells: select human umbilical cord mesenchymal stem cells with good growth status and negative microbial detection, P2-P8 human umbilical cord mesenchymal stem cells, according to 3-5*10 4 / cm 2 The initial density is inoculated in cell culture flasks or cell culture dishes, and the culture medium is a culture system containing fetal bovine serum with a volume ratio concentration of 5-15% in the basal medium;

[0021] (2) After 2-4 days of inoculation and culture, the cell fusion rate reaches 80-95%, and the cell growth state is good. Discard all the culture supernatant, and wash the cells 2-4 times with medical compound electrolyte injection, and then add compound electrolyte solution , so that the height of the...

Embodiment example 1

[0031] Patient Li, 40 years old, due to busy work and high pressure, experienced loss of libido and frequency of sexual life one year ago, and early symptoms of erectile dysfunction. The average frequency of sexual life in the first 3 months of treatment was 1 time / Two of them did not complete the sexual life because the penis could not get a normal erection; 5 times the penis failed to maintain an erection before ejaculation during sexual intercourse, and ejaculation failed; the other 7 times completed the sexual intercourse, and the average time was less than 3 minutes. According to the "International Erectile Function Index" self-test score is 12 points, which belongs to mild erectile dysfunction.

[0032] Preparation of joint preparations:

[0033] (1) Cultivation of human umbilical cord mesenchymal stem cells: select human umbilical cord mesenchymal stem cells with good growth status and negative microbial detection, and P4 human umbilical cord mesenchymal stem cells ac...

Embodiment example 2

[0041] Patient Zhang, 52 years old, had decreased frequency of sexual life and symptoms of erectile dysfunction. The average frequency of sexual life in the first 6 months of treatment was 1 time / two weeks, of which 2 times he did not complete the sexual life because the penis could not get a normal erection; 4 During sexual intercourse, the penis failed to maintain an erection before ejaculation, and ejaculation failed; the other 6 times completed the sexual intercourse, and the average time was less than 3 minutes. According to the "International Erectile Function Index" self-test score is 8 points, which belongs to moderate erectile dysfunction.

[0042] Preparation of joint preparations:

[0043] (1) Cultivation of human umbilical cord mesenchymal stem cells: select P6 human umbilical cord mesenchymal stem cells with good growth status and negative microbial detection, according to 3*10 4 / cm 2 The initial density is inoculated in 20 bottles of T-175 cell culture flasks,...

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Abstract

The invention discloses a combined preparation of umbilical cord mesenchymal stem cells and exosomes for treatment of erectile dysfunction, and a preparing method and a drug administration method thereof. The combined preparation includes umbilical cord mesenchymal stem cells and an umbilical cord mesenchymal stem cell exosome concentrate obtained by starvation culture. Through the method of starvation culture of the umbilical cord mesenchymal stem cells, the secretion of bioactive substances is promoted. The bioactive substances secreted by the umbilical cord mesenchymal stem cells are recovered in a form of exosomes, the recovery efficiency is greatly improved and the recovery method is more convenient. The exosomes secreted by the umbilical cord mesenchymal stem cells are collected andconcentrated by a method of gradient filtration with biological films with different pore sizes, the process of exosome recovery is simplified, and the purity of the exosomes is improved.

Description

technical field [0001] The invention relates to a combined preparation of umbilical cord mesenchymal stem cells and exosomes thereof, in particular to a combined preparation of umbilical cord mesenchymal stem cells and exosomes for treating erectile dysfunction, a preparation method and an administration method thereof. Background technique [0002] Erectile dysfunction (erectile dysfunction, ED) is the most common male sexual dysfunction, refers to the persistent inability to achieve or maintain a sufficient erection of the penis to complete a satisfactory sexual life, and the course of the disease is more than 3 months. ED can generally be divided into organic ED, surgical or traumatic ED, mental ED, penile lesions, etc. Conventional treatment methods can be treated with oral medication, but the drug treatment is highly dependent and cannot achieve the goal of a radical cure. Umbilical cord mesenchymal stem cells are adult stem cells, which have multiple functions such as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/51C12N5/0775A61P15/10A61P15/00
CPCC12N5/0665A61K35/51A61K9/0019A61K9/0021A61P15/10A61P15/00C12N2509/00A61K2300/00
Inventor 张冰晶韩洪起鲁振宇牟鑫煜徐悦
Owner 天津欣普赛尔生物医药科技有限公司
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