Use of bacteriophage ah10-phage-qy01 in the preparation of drugs for the treatment or prevention and control of bacterial diseases in aquaculture

A bacteriophage and application technology, applied in the field of aquaculture bacterial disease prevention and treatment, can solve the problems of easy-to-get and difficult-to-treat sepsis, difficult prevention and control, strong drug resistance, etc., and achieve good inhibitory effect, strong bacteriostatic effect and high specificity Effect

Inactive Publication Date: 2021-07-13
SHANGHAI OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Aiming at the current bacterial fish disease, especially the highly pathogenic bacterial disease Aeromonas Hydrophila (Aeromonas Hydrophila) infection caused by sepsis is easy to get and difficult to treat, strong drug resistance and difficult to prevent and control, the purpose of the present invention is to provide Application of Aeromonas hydrophila phage AH10-Phage-QY01 in the preparation of drugs for the treatment or prevention and control of bacterial diseases in aquaculture

Method used

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  • Use of bacteriophage ah10-phage-qy01 in the preparation of drugs for the treatment or prevention and control of bacterial diseases in aquaculture
  • Use of bacteriophage ah10-phage-qy01 in the preparation of drugs for the treatment or prevention and control of bacterial diseases in aquaculture

Examples

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Embodiment

[0024] Example: Screening, Isolation and Purification of Aeromonas hydrophila Phage AH10-Phage-QY01

[0025] Taking advantage of the ubiquity of phages and their high specificity to host bacteria, in the following examples, the corresponding phages were screened from the natural environment for various aquaculture pathogenic bacteria and used to treat the aquaculture bacteria caused by the pathogenic bacteria sick. The specific steps are:

[0026] Take 50ml of sampling sample and centrifuge at 4000rpm / min for 30min.

[0027] Aspirate 10ml of the supernatant, filter and sterilize with a 0.22um filter.

[0028] Add 25ml of 3 times concentrated LB liquid medium into a 200ml Erlenmeyer flask, cool to room temperature, add the filtrate into the Erlenmeyer flask, add 1ml of host bacteria, and add 0.5M calcium chloride to a final concentration of 0.1mM at the same time, Cultivate at 28 degrees for 12-14 hours.

[0029] Take out 3ml of the culture solution and put it into a 5ml st...

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Abstract

The invention discloses the use of Aeromonas hydrophila bacteriophage AH10-Phage-QY01 in preparing a drug for treating or preventing and controlling aquaculture bacterial diseases, and belongs to the technical field of aquaculture bacterial disease prevention and control. The bacteriophage AH10-Phage-QY01 is deposited in the China Center for Type Culture Collection, the deposit number is CCTCC NO: M2019109, and also includes the use of the bacteriophage AH10-Phage-QY01 in the preparation of bacterial septicemia caused by Aeromonas hydrophila, which can be Used alone or in combination with antibiotics. The present invention utilizes the ubiquity of phage and its high specificity to host bacteria to screen out Aeromonas hydrophila phage AH10-Phage-QY01 from the natural environment for the first time against pathogenic bacteria, which is used to inhibit and lyse pathogenic bacteria, To treat or prevent bacterial fish diseases such as bacterial sepsis caused by the pathogenic bacteria.

Description

technical field [0001] The invention belongs to the technical field of prevention and control of aquaculture bacterial diseases, and in particular relates to Aeromonas hydrophila phage AH10-Phage-QY01 and its use in preparing medicines for treating or preventing and controlling aquaculture bacterial diseases. Background technique [0002] Bacterial fish disease is the main disease of freshwater aquaculture industry. During the epidemic period, it not only leads to the death of a large area of ​​cultured species and fish eggs, but also makes the cultured products lose their commercial value, causing serious economic losses. Bacterial fish diseases are easy to get, difficult to treat, and easy to produce drug resistance. At present, due to the extensive use of antibiotics, various aquatic pathogens are becoming more and more drug-resistant, and the prevention and control of bacterial fish diseases is becoming more and more difficult. . [0003] Sepsis caused by Aeromonas Hydr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K35/76A61P31/04C12R1/92
CPCA61K35/76A61P31/04C12N7/00C12N2795/00021C12N2795/00032
Inventor 吕利群徐宁王浩
Owner SHANGHAI OCEAN UNIV
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