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Method for simultaneously positioning multiple genes

A gene and genotype technology, applied in the field of locating multiple genes at the same time, can solve the problems of low utilization rate and high cost of the group, and achieve the effects of reducing phenotypic interference, improving utilization efficiency, and reducing complexity

Inactive Publication Date: 2019-08-13
HENAN XINXIANG ACADEMY OF AGRI SCI
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

The above method has played an important role in gene mapping, but only one gene can be mapped for each population constructed, the utilization rate of the population is low, and the cost is high

Method used

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  • Method for simultaneously positioning multiple genes
  • Method for simultaneously positioning multiple genes
  • Method for simultaneously positioning multiple genes

Examples

Experimental program
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Effect test

Embodiment 1

[0035] A method for locating multiple genes simultaneously, comprising the steps of:

[0036] (1) Build groups:

[0037] A wrinkled-skinned pickled pepper mutant 242 without stem hair was used as the female parent, and the sweet pepper variety Xinke 17 was used as the male parent to cross, and F1 was obtained, and the BC1F1 population was obtained by backcrossing with Xinke 17, and F1 was self-crossed to obtain F2 groups.

[0038] For the phenotypes of sweet pepper Xinke 17 and pickled pepper mutant 242, see image 3 .

[0039] (2) Using molecular markers to assist genotyping:

[0040]Select the molecular markers in the pepper KL-DH genetic map, see the literature "Development of simple sequence repeat markers and construction of a high-density linkage map of Capsicum annuum" for details, randomly select a BC1F1 individual plant B-7 and any F2 individual plant F2 -6, taking B-7 and F2-6 as samples, screening co-dominant polymorphic markers. Results Three co-dominant SSR p...

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Abstract

The invention relates to a method for simultaneously positioning multiple genes. The method comprises the steps of creating a BC1F1 group and an F2 group, screening a codominant polymorphic molecule mark, performing genotyping on the F2 group by means of the molecule mark, then classifying the materials with the same genotype to one group, investigating the phenotype difference between different groups and performing statistical analysis, performing verification by means of a hybrid genotype F2 single plant progeny, namely positioning a certain phenotype trait gene next to the specific molecule mark. The method of the invention has advantages of the positioning the quantitative character and qualitative character, improving group utilization efficiency and realizing large-scale gene positioning by emans of a phenomics-related facility.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for simultaneously locating multiple genes. Background technique [0002] Gene mapping is the determination of the position of a gene on a chromosome. In the early days, the two-point test and the three-point test were the main methods to determine the distance and order between genes, and the distance between genes was represented by the exchange value. Later, according to chromosomal aneuploidy, deletion and translocation, the chromosomal location of genes was developed, and the genetic map was constructed by using cytological markers such as karyotype and band type of chromosomes. After the invention of DNA molecular markers, due to its various advantages, it has been widely used in the fields of genetic map construction and gene positioning. DNA molecular markers can be divided into dominant markers and co-dominant markers. Co-dominant markers can distingui...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B20/30G16B30/10C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/156G16B20/30G16B30/10
Inventor 焦禹顺刘贺娟陈昊放孙强郭志伟任福森
Owner HENAN XINXIANG ACADEMY OF AGRI SCI
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