A kind of identification method of castor bean leaf
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An identification method, castor leaf technology, applied in the field of plant identification, can solve the problems of castor leaf health damage and reduced accuracy
Inactive Publication Date: 2020-07-07
GUIZHOU MEDICAL UNIV
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Problems solved by technology
Therefore, it is necessary to identify castor leaves, and only rely on manual observation and taste identification, which has the determination that the accuracy is reduced due to excessive reliance on experience and that eating castor leaves can easily cause health damage
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Embodiment 1
[0069] Preparation of the test solution: Weigh 1 g of the test powder, add 10 mL of absolute ethanol, soak for 20 minutes, filter, and take the filtrate as the test solution;
[0070] Preparation of reference substance solution: Weigh 1 g of castor leaf reference medicinal material powder, add 10 mL of absolute ethanol, soak in cold for 20 min, filter, and take the filtrate as reference substance solution;
[0071] Thin-layer chromatography plate: silica gel G thin-layer chromatography plate;
[0072] Spotting: spot 1 μL of the test solution and the reference solution respectively;
[0081] Preparation of the test solution: take 2 g of the test powder, add 30 mL of absolute ethanol, soak for 30 min, filter, and take the filtrate as the test solution;
[0082] Preparation of reference substance solution: Weigh 2 g of castor leaf reference medicinal material powder, add 30 mL of absolute ethanol, cold soak for 30 min, filter, and take the filtrate as reference substance solution;
[0083] Thin-layer chromatography plate: silica gel G thin-layer chromatography plate;
[0084] Spotting: spot 1.5 μL of the test solution and the reference solution respectively;
[0087] Expansion method: upward expansion, the expansion distance is 10cm;
[0088] Unfolding environment: 30°C, RH60%;
[0089] Inspection: inspection under visible light;
[0090] Chromatogram identification: In the chroma...
Embodiment 3
[0092] Preparation of the test solution: Weigh 3 g of the test powder, add 20 mL of absolute ethanol, soak in cold for 25 min, filter, and take the filtrate as the test solution;
[0093] Preparation of reference substance solution: Weigh 3 g of castor leaf reference medicinal material powder, add 20 mL of absolute ethanol, cold soak for 25 minutes, filter, and take the filtrate as reference substance solution;
[0094] Thin-layer chromatography plate: silica gel G thin-layer chromatography plate;
[0095] Spotting: spot 1 μL of the test solution and the reference solution respectively;
[0097] Color developer: phosphomolybdic acid solution, baked at 108°C until the spots are clear.
[0098] Expansion method: upward expansion, the expansion distance is 12cm;
[0099] Unfolding environment: 35°C, RH30%;
[0100] Inspection: inspection under visible light;
[0101] Chromatogram identification: In...
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Abstract
The invention belongs to the technical field of plant identification, and particularly relates to a castor bean leaf identification method. When the microscopic characteristics of a test article are castor bean leaf microscopic characteristics after the test article is subjected to microscopic identification, thin layerchromatography identification is then carried out. The thin layerchromatography identification comprises the following steps: 1) a test article solution and a reference substance solution are prepared respectively; and 2) the test article solution and the reference substance solution are sucked to be dropped on the same thin layerchromatography plate, with a petroleumether-ethyl acetate-methanol as a developer, developing, taking out, air drying, spraying of a phosphomolybdic acidtest solution, heating to enabling a spot to develop a color clearly and placing under visible light for inspection are carried out, when fluorescent spots of the same color are developed at the corresponding positions of the chromatogram of the test article solution and the chromatogram of the reference substance solution, the test article is judged to contain a castor bean leaf. The method disclosed in the invention is scientific and reasonable, has high accuracy and high safety, avoids human harm, and has the advantages of strong stability, low identification cost and short identification time.
Description
technical field [0001] The invention belongs to the technical field of plant identification, in particular to an identification method of castor leaves. Background technique [0002] Castor is an annual stout herb or herbaceous shrub, up to 5 meters high; branchlets, leaves and inflorescences are usually covered with hoarfrost, and the stems are juicy. Leaf outline nearly round, length and width up to 40 cm or more, palmately 7-11 lobed, lobes almost to the middle, lobes ovate-oblong or lanceolate, apex acute or acuminate, margin serrate . Veins obvious; petioles thick, hollow, up to 40 cm long, with 2 discoid glands at the top and discoid glands at the base; stipules long triangular, 2-3 cm long, caducous. Racemes or panicles, 15-30 cm or longer; bracts broadly triangular, membranous, caducous; male flowers: calyx lobes ovate-triangular, 7-10 mm long; stamen bundles numerous; female flowers: sepals ovate Ovary lanceolate, 5-8 mm long, withered; ovary ovate, about 5 mm in...
Claims
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Application Information
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