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Molecular marker composition for identifying eggplant germplasm resources and application of molecular marker composition

A technology of molecular markers and eggplants, applied in the field of molecular biology, can solve the problems of difficult and inaccurate identification

Active Publication Date: 2019-09-13
VEGETABLE & FLOWER INST JIANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the identification methods of eggplant germplasm resources are mainly morphological methods, which are difficult and inaccurate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Example 1 The establishment and application of the method for identifying eggplant germplasm resources using the combination of SNP and InDel markers

[0097] 1. Experimental materials

[0098] The experimental materials include 55 eggplant materials: Xiangzao eggplant, rouge eggplant (V06B0149), dog tail eggplant (V06B0458), black beauty (V06B0541), Vetrioe (V06B0543), red skin cold eggplant (V06B0552), Nanfeng Xian eggplant (V06B0556 ), Shexian round white eggplant (V06B0978), Fujiqiao purple eggplant (V06B1046), oil tank eggplant (V06B0357), purple round eggplant (V06B0940), five-claw eggplant (V06B1256), bamboo silk eggplant, six-leaf eggplant (V06B0039) , white eggplant (V06B0066), purple long eggplant (V06B0092), eel head eggplant (V06B1231), Yongan silver bead eggplant (V06B0108), white elephant eggplant (V06B0117), Wangbu purple long eggplant (V06B0150), black oil eggplant ( V06B0476), flat eggplant (V06B0841), six-leaf eggplant (V06B1027), black five-leaf eggp...

Embodiment 2

[0159] Embodiment 2 identifies different eggplant materials

[0160] 1. Identification of eggplant material Quanzhou white eggplant (V06B0101)

[0161] Collect the tender leaves of eggplant material Quanzhou Baipi Eggplant (V06B0101), extract its genomic DNA according to the method of Example 1, and carry out PCR amplification to it with 9 primer sets consisting of SEQ ID NO:1~SEQ ID NO:18 Amplified and sequenced separately, according to the method of Example 1, a molecular marker combination consisting of 17 sites from SI-1 to SI-17 was constructed: G SI-1 A SI-2 T SI-3 G SI-4-SI- 5 C SI-6 G SI-7 C SI-8-SI-9 C SI-10 T SI-11 C SI-12 G SI-13 G SI-14-SI-15 T SI-16 T SI-17, comparing the molecular marker combination with the "molecular marker combination XII" comparison card in Example 1, and found that the molecular marker combination does not exist in the comparison card.

[0162] 2. Identification of eggplant material Shijie long eggplant

[0163] Collect the t...

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PUM

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Abstract

The invention provides a molecular marker composition for identifying eggplant germplasm resources and application of the molecular marker composition. The molecular marker composition consists of 17SNP and InDel markers, and primer sequences for amplifying the molecular marker composition are shown in SEQ ID NO:1-18 respectively. The molecular marker composition expands the available marker siterange of eggplants, and the method for indentifying 12 parts of eggplant germplasm resources by the molecular marker composition is simple to operate, high in accuracy and reliable in result. The molecular marker composition has important theoretical and application values in aspects of eggplant germplasm resource identification, DNA fingerprint drawing, variety protection and management and thelike.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a combination of molecular markers for identification of eggplant germplasm resources and its application. Background technique [0002] Eggplant (Solanum melongena L.) is an important cultivated crop of Solanaceae Solanaceae. It contains saponin, vitamin P, calcium, phosphorus and other nutrients. important vegetable crops in , Middle East, Near East, Southern Europe and Africa. Eggplant originated in tropical Asia, and ancient India was its earliest domestication place. China, as a large production and consumption country of eggplant, is considered to be the second origin of eggplant. [0003] After many years of domestication, the genetic background of eggplant cultivars is relatively narrow, and many germplasm resources have similar phenotypic traits and genetic backgrounds, which makes the identification of germplasm resources more difficult. At present, the identification...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 袁欣捷方荣陈学军周坤华雷刚黄月琴
Owner VEGETABLE & FLOWER INST JIANGXI ACADEMY OF AGRI SCI
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