Fixing needle for mouse embryo amnion cavity microinjection and preparing method thereof

Abstract

The invention relates to a fixing needle for mouse embryo amnion cavity microinjection and a preparing method thereof, and belongs to the technical field of medical apparatuses. The technical problems are solved that gel micropores applied in a mouse amnion cavity injection technology do not easily fix embryos, operation cannot be carried out under a high-power lens, and the difficulty in the injection process is large. According to the scheme, the fixing needle comprises an inclined part and a horizontal part, one end of the inclined part is fixedly connected with one end of the horizontal part, the inclined part is communicated with the horizontal part, the end portion of the free end of the horizontal part is aslant provided with a cutting edge with an upward opening, and the middle of the horizontal part is further provided with a reducing part which is sunken towards the inner side of the horizontal part. Through the process of needle drawing, needle grinding and needle forging, the fixing needle is prepared. The fixing needle is low in cost, the preparing process is simple and feasible, the prepared fixing needle can simplify the operation process, in the process of transferring the embryos after injection, the embryos are not easily influenced by the external environment in the fixing needle, and the fixing needle can better protect the embryos.

Description

technical field [0001] The invention belongs to the technical field of mouse amniotic cavity injection, and specifically relates to a fixing needle for microinjection of mouse embryo amniotic cavity and a preparation method thereof. Background technique [0002] The anterior amniotic cavity has been formed on the 6th day of mouse development after fertilization. At 6.5 days, cells, exogenous DNA and other substances can be injected for cell migration and development studies. Microinjection into the amniotic cavity has been proven to be useful for exploring the developmental results of changing gene expression. And it is valuable to study cell lineage or migration during the second half of mouse embryonic development (from gestational embryonic day 9.5). [0003] The existing fixation device for microinjection into the amniotic cavity is a gel with small holes. The method is to dig a small hole after the agarose gel is prepared, put the mouse embryo into the small hole, plac...

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the deficiencies in the prior art and solve the technical problems that the gel holes used in the mouse amniotic cavity injection technology are not easy to fix the embryo, cannot be operated under a high-power microscope, and the injection process is difficult, the invention provides a method for Fixing needle for mouse embryo amniotic cavity microinjection and preparation method thereof

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