Separating method for living cells in ship ballast tank sediments

A separation method and sediment technology, applied in the direction of animal cells, etc., can solve the problems of long time consumption and restricted development, and achieve the effect of simple steps.

Pending Publication Date: 2014-08-27
山东恒诚检测科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method takes a long time and requires inspection and quarantine personnel to have rich experience in biological classification, which seriously restricts the development of inspection and quarantine of foreign organisms in the ballast tank sediments of ships.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for rapidly separating living cells from a ship's ballast tank sediment sample docked at Zhifu Port, Yantai, comprising the steps of:

[0025] (1) Collect the ballast tank sediment of the ship, get 5 grams of sediment and place it in a petri dish;

[0026] (2) flush sediment with 100 milliliters of 0.45 micron seawater filter, adopt the first sieve silk filter of 200 micron aperture, preserve the liquid after flushing and filtering with plastic bottle;

[0027] (3) The liquid preserved in the plastic bottle is filtered by the second sieve silk with 50 micron aperture, and the residue on the second sieve silk is retained;

[0028] (4) Rinse the residue on the second sieve silk with 100 milliliters of saturated sucrose solution with a concentration of 200-220 grams of sucrose / 100 milliliters of distilled water into the centrifuge tube;

[0029] (5) Centrifuge the centrifuge tube for 3 minutes under the condition of 1000rpm on a desktop centrifuge;

[0030] (6) ...

Embodiment 2

[0033] The rapid separation of living cells from the ballast tank sediment samples of ships docked at Longkou Port in Yantai includes the following steps:

[0034] (1) Collect the ballast tank sediment of the ship, get 5 grams of sediment and place it in a petri dish;

[0035] (2) flush sediment with 100 milliliters of 0.45 micron seawater filter, adopt the first sieve silk filter of 200 micron aperture, preserve the liquid after flushing and filtering with plastic bottle;

[0036] (3) the liquid preserved in the plastic bottle is adopted to filter the second sieve of 40 micron aperture, and the residue on the second sieve is retained;

[0037] (4) Rinse the residue on the second sieve silk with 100 milliliters of saturated sucrose solution with a concentration of 200-220 grams of sucrose / 100 milliliters of distilled water into the centrifuge tube;

[0038] (5) Centrifuge the centrifuge tube for 5 minutes under the condition of 1200rpm on a desktop centrifuge;

[0039] (6) a...

Embodiment 3

[0042] The rapid separation of living cells from the ballast tank sediment samples of ships docked at Dongying Port includes the following steps:

[0043] (1) Collect the ballast tank sediment of the ship, get 5 grams of sediment and place it in a petri dish;

[0044] (2) flush sediment with 100 milliliters of 0.45 micron filter seawater, adopt the first sieve silk filter of 250 micron aperture, preserve the liquid after flushing and filtering with plastic bottle;

[0045] (3) The liquid preserved in the plastic bottle is filtered by the second sieve of 30 micron aperture, and the residue on the second sieve is retained;

[0046] (4) Rinse the residue on the second sieve silk with 100 milliliters of saturated sucrose solution with a concentration of 200-220 grams of sucrose / 100 milliliters of distilled water into the centrifuge tube;

[0047] (5) Centrifuge the centrifuge tube for 3 minutes under the condition of 1200rpm on a desktop centrifuge;

[0048] (6) absorb all the s...

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PUM

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Abstract

The invention discloses a separating method for living cells in ship ballast tank sediments. The method comprises the steps that (1) the ship ballast tank sediments are collected and placed into a petri dish; (2) filtrated sea water is used for washing the sediments, and a first bolting cloth is adopted for filtration, and liquid obtained after washing is reserved; (3) the reserved liquid is filtered by a second bolting cloth, and residues on the second bolting cloth are reserved; (4) a saturated sucrose solution is used for washing the residues on the second bolting cloth to a centrifuge tube; (5) the centrifuge tube is centrifuged; (6) supernatant liquid on the centrifuge tube is absorbed, a third bolting cloth is adopted for filtration, and residues on the third bolting cloth are reserved; (7) the filtered sea water is used for rinsing the residues on the third bolting cloth to a petri dish, separation of the living cells is achieved, and the living cells are observed under a microscope. The method is easy to implement, convenient and easy to operate, and capable of achieving rapid separation.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for separating living cells in sediments of ship ballast tanks. Background technique [0002] Alien biological invasion poses a serious threat to my country's ecological environment and social development, causing major economic losses. Resisting alien biological invasion and protecting national border security are the urgent duties and obligations of entry-exit inspection and quarantine. With the rapid development of the maritime shipping industry, the problem of marine biological invasion caused by ship ballast water and sediment is becoming more and more serious. Marine biological invasion will destroy the structure and function of the marine ecosystem and cause certain economic losses. The marine organisms carried by ships' ballast water and sediments can survive for several months and enter other waters with the discharge of ballast water, which provides a convenient me...

Claims

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Application Information

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IPC IPC(8): C12N5/07
Inventor 黄强
Owner 山东恒诚检测科技有限公司
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