Separating method for living cells in ship ballast tank sediments
A separation method and sediment technology, applied in the direction of animal cells, etc., can solve the problems of long time consumption and restricted development, and achieve the effect of simple steps.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0024] A method for rapidly separating living cells from a ship's ballast tank sediment sample docked at Zhifu Port, Yantai, comprising the steps of:
[0025] (1) Collect the ballast tank sediment of the ship, get 5 grams of sediment and place it in a petri dish;
[0026] (2) flush sediment with 100 milliliters of 0.45 micron seawater filter, adopt the first sieve silk filter of 200 micron aperture, preserve the liquid after flushing and filtering with plastic bottle;
[0027] (3) The liquid preserved in the plastic bottle is filtered by the second sieve silk with 50 micron aperture, and the residue on the second sieve silk is retained;
[0028] (4) Rinse the residue on the second sieve silk with 100 milliliters of saturated sucrose solution with a concentration of 200-220 grams of sucrose / 100 milliliters of distilled water into the centrifuge tube;
[0029] (5) Centrifuge the centrifuge tube for 3 minutes under the condition of 1000rpm on a desktop centrifuge;
[0030] (6) ...
Embodiment 2
[0033] The rapid separation of living cells from the ballast tank sediment samples of ships docked at Longkou Port in Yantai includes the following steps:
[0034] (1) Collect the ballast tank sediment of the ship, get 5 grams of sediment and place it in a petri dish;
[0035] (2) flush sediment with 100 milliliters of 0.45 micron seawater filter, adopt the first sieve silk filter of 200 micron aperture, preserve the liquid after flushing and filtering with plastic bottle;
[0036] (3) the liquid preserved in the plastic bottle is adopted to filter the second sieve of 40 micron aperture, and the residue on the second sieve is retained;
[0037] (4) Rinse the residue on the second sieve silk with 100 milliliters of saturated sucrose solution with a concentration of 200-220 grams of sucrose / 100 milliliters of distilled water into the centrifuge tube;
[0038] (5) Centrifuge the centrifuge tube for 5 minutes under the condition of 1200rpm on a desktop centrifuge;
[0039] (6) a...
Embodiment 3
[0042] The rapid separation of living cells from the ballast tank sediment samples of ships docked at Dongying Port includes the following steps:
[0043] (1) Collect the ballast tank sediment of the ship, get 5 grams of sediment and place it in a petri dish;
[0044] (2) flush sediment with 100 milliliters of 0.45 micron filter seawater, adopt the first sieve silk filter of 250 micron aperture, preserve the liquid after flushing and filtering with plastic bottle;
[0045] (3) The liquid preserved in the plastic bottle is filtered by the second sieve of 30 micron aperture, and the residue on the second sieve is retained;
[0046] (4) Rinse the residue on the second sieve silk with 100 milliliters of saturated sucrose solution with a concentration of 200-220 grams of sucrose / 100 milliliters of distilled water into the centrifuge tube;
[0047] (5) Centrifuge the centrifuge tube for 3 minutes under the condition of 1200rpm on a desktop centrifuge;
[0048] (6) absorb all the s...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com