Primer for detecting cucumber mosaic virus-passiflora isolates and application thereof

Abstract

The invention provides primers for detecting cucumber mosaic virus-passiflora isolates. A primer pair in the primers includes primers CMV-657F and CMV-657R for identifying the cucumber mosaic virus-passiflora isolates, and the other primer pair in the primers includes primers CMV-174F and CMV-174R used for real-time fluorescent quantitative RT-PCR of the cucumber mosaic virus-passiflora isolates.The invention also provides application of the primers to quantitative detection of cucumber mosaic virus-passiflora isolates. The quantitative detection method combining the two primer pairs has highsensitivity, high specificity and accurate detection result, is used for early identification of passiflora virus-free seedlings and field infected plants and virus content quantitative analysis, andprovides technical support for seedling breeding and promotion of passiflora virus-free improved varieties. The method can accurately quantify the cucumber mosaic virus-passiflora isolates in the sample within 4-5 hours, thus saving time, simplifying the experimental steps, facilitating corresponding remedial measures before the onset of the plant disease and preventing huge economic losses caused by wide spreading of the virus among crops.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a primer for detecting Cucumber Mosaic Virus Passiflora isolate and an application thereof. Background technique [0002] Cucumovirus (Cucumovirus) is a member of the Bromoviridae family (Bromoviridae). It is a three-part single-stranded RNA virus, including 4 RNA segments, which encode different proteins, and the virus particles are equiaxed. icosahedron. Cucumber mosaic virus (CMV) has a wide range of hosts and can infect more than 1200 kinds of plants. More than 100 CMV strains or isolates have been reported. In the 1980s and 1990s, cucumber mosaic virus had caused great harm to the passionflower industry in Taiwan, Fujian, Guangdong and other provinces and regions of my country. In August 2016, plants suspected to be infected by cucumber mosaic virus were also found in Guizhou Province, which poses a potential threat to industrial development. The bree...

AI Technical Summary

Problems solved by technology

Identification of indicator plants takes a long time and is limited by environmental conditions

The observation results of electron microscope technology are intuitive and accurate, but the equipment is expensive, and the production and operation techniques are complicated and difficult to master

Serological methods are fast and intuitive, but the accuracy of the results often depends on the quality of the antiserum, and cannot detect unstable viruses

Ordinary RT-PCR has less sample volume and strong specificity, but its sensitivity is not as good as real-time fluorescent quantitative RT-PCR, and it cannot quantitatively analyze the virus content

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