Method for detecting quality level of traditional Chinese medicine
A detection method and quality grade technology, applied in the direction of alternative drugs, measuring devices, material inspection products, etc., can solve the problems of inability to evaluate the quality of Chinese medicine grades, etc.
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Examples
Embodiment 1
[0079] Embodiment 1 Chinese medicine cassia twig quality grade detection method
[0080] (1) Sample preparation: take about 0.3g of cassia twig powder (passed through a No. 4 sieve), accurately weigh it, put it in a stoppered Erlenmeyer flask, add 50mL of methanol precisely, seal it tightly, weigh it, and ultrasonically treat it (power 140w, Frequency 42kHz) for 30 minutes, let cool, weigh again, make up the lost weight with methanol, shake well, filter, take the filtrate to obtain the sample solution.
[0081] (2) Adopt ultra-high liquid chromatography to collect the integral data of cassia twig fingerprint spectrum: adopt ultra-high liquid chromatography, under the characteristic wavelength 254nm of the main component of cassia twig, use octadecylsilane bond silica gel as filler; use 0.1 % formic acid-water solution is the mobile phase A, acetonitrile is the mobile phase B, and the gradient elution is carried out according to the following conditions: time 0-2min, the volume...
Embodiment 2
[0096] Embodiment 2 Chinese medicine salvia miltiorrhiza quality grade detection method
[0097] (1) Sample preparation: Take about 0.3g of Salvia miltiorrhiza powder (passed through a No. 4 sieve), accurately weigh it, put it in a stoppered Erlenmeyer flask, add 50mL of methanol precisely, seal it tightly, weigh it, and ultrasonically treat it (power 140w, frequency 42kHz) for 30 minutes, let it cool down, weighed again, made up the lost weight with methanol, shaked well, filtered, and took the filtrate to obtain the sample solution.
[0098] (2) Using ultra-high liquid chromatography to collect the integral data of Danshen fingerprints: using optimized ultra-high liquid chromatography, under the characteristic wavelength of the main components of Danshen at 270nm, octadecylsilane bonded silica gel is used as filler; 0.1 % formic acid-water solution is the mobile phase A, acetonitrile is the mobile phase B, and the gradient elution is carried out according to the following co...
Embodiment 3
[0113] Embodiment 3 Chinese medicine Achyranthes bidentata quality grade detection method
[0114] (1) Sample preparation: Take about 0.3g of Achyranthes bidentata powder (passed through a No. 4 sieve), accurately weigh it, put it in a stoppered Erlenmeyer flask, add 50mL of methanol precisely, seal it tightly, weigh it, and perform ultrasonic treatment (power 140w, Frequency 42kHz) for 30 minutes, let cool, weigh again, make up the lost weight with methanol, shake well, filter, take the filtrate to obtain the sample solution.
[0115] (2) Collect the integrated data of Achyranthes bidentata fingerprints by ultra-high liquid chromatography: using optimized ultra-high liquid chromatography, under the characteristic wavelength of 250nm of the main components of Achyranthes bidentata, use octadecylsilane bond silica gel as filler; With 0.1% formic acid-water solution as mobile phase A and acetonitrile as mobile phase B, carry out gradient elution according to the following condit...
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Description
Claims
Application Information
- IPC
- G01N30/02; G01N30/06; G01N21/25; G01N30/86
- CPC
- G01N30/02; G01N30/06; G01N21/25; G01N30/8686; G01N30/8696; G01N2030/047; G01N33/15; G16H20/90
- Inventors
- 刘妍如; 唐志书