A kind of Procambarus clarkii type I lysozyme glysi2 gene, its encoded glysi2 protein and application thereof

A pgex-4t-1-lysi2, protein technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problem of very few research and reports on the lysozyme of Crayfish, achieve strong inhibitory effect, inhibit replication horizontal effect

Active Publication Date: 2022-01-28
HUAZHONG AGRI UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0005] At present, there are very few studies and reports on lysozyme of Procambarus clarkii

Method used

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  • A kind of Procambarus clarkii type I lysozyme glysi2 gene, its encoded glysi2 protein and application thereof
  • A kind of Procambarus clarkii type I lysozyme glysi2 gene, its encoded glysi2 protein and application thereof
  • A kind of Procambarus clarkii type I lysozyme glysi2 gene, its encoded glysi2 protein and application thereof

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Embodiment Construction

[0033] The principles and features of the present invention are described below, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0034] The invention provides a Procambarus clarkii type I lysozyme gLysi2 gene, the nucleotide sequence of the gLysi2 gene is SEQ ID NO.1.

[0035] The above-mentioned Procambarus clarkii type I lysozyme gLysi2 gene can be widely used in the field of combating white spot syndrome of prawns.

[0036] A gLysi2 protein can be prepared by using the gLysi2 gene coded by the above-mentioned Procambarus clarkii type-i lysozyme, and the amino acid sequence of the gLysi2 protein is SEQ ID NO.2. Its SDS-PAGE analysis chart is shown in figure 1 .

[0037] The above-mentioned gLysi2 protein has extremely wide application in the field of treatment of white spot syndrome of prawns. By directly injecting the above-mentioned protein into the shrimp, the shrimp can obtain t...

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Abstract

The present invention discovers a Procambarus clarkii type I lysozyme gLysi2 gene for the first time, and the nucleotide sequence of the gLysi2 gene is SEQ ID NO.1. The present invention utilizes the gene code to prepare a gLysi2 protein, and the amino acid sequence of the gLysi2 protein is SEQ ID NO.2. The invention also provides the application of the above-mentioned genes and proteins in the field of prevention and treatment of white spot syndrome of prawns. The invention has an effective antiviral effect, has a strong inhibitory and killing effect on the white spot syndrome baculovirus, and can effectively solve the problems of immunity and treatment of the white spot syndrome.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a Procambarus clarkii type I lysozyme gLysi2 gene, a gLysi2 protein encoded by it and an application thereof. Background technique [0002] Procambarus clarkii, also known as freshwater crayfish, is shaped like a shrimp and has a hard shell; the adult is about 5.6 to 11.9 cm long. Procambarus clarkii is a freshwater economical shrimp, which is widely welcomed by people because of its delicious meat. Because of its omnivorous nature, fast growth rate and strong adaptability, it forms an absolute competitive advantage in the local ecological environment. Its food range includes aquatic plants, algae, aquatic insects, animal carcasses, etc. In recent years, Procambarus clarkii has become an important economic breeding species in my country, with a huge output. [0003] Shrimp white spot syndrome is a comprehensive disease caused by white spot syndrome baculovirus. The wh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/56C12N9/36C12N15/70C12N15/62C07K19/00A61K38/47A61P31/20C12R1/19
CPCC12N9/2462C12N15/70A61P31/20C12Y302/01017C07K2319/23A61K38/00
Inventor 兰江风张英豪李通曹晓彤殷成明顾泽茂
Owner HUAZHONG AGRI UNIV
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