Three-dimensional microfluidic devices and methods for high-throughput microdroplet generation

Abstract

The invention provides a three-dimensional microfluidic device and method for generating high-flux micro-droplets. The device comprises a closed type flow channel structure formed by bonding a bottom-layer PDMS flow channel, a middle-layer PDMS flow channel and an upper-layer PDMS flow channel; the closed type flow channel structure is provided with a dispersed phase inlet connector, a continuousphase inlet connector and a collection outlet connector; the method comprises the following steps: fixing the three-dimensional microfluidic device on an objective table of a microscope and starting an injection pump; firstly, adjusting a dispersed phase and a continuous phase to corresponding flow speeds through the injection pump; and shearing the dispersed phase into the micro-droplets by the continuous phase on each T-shaped flow channel array for generating the micro-droplets in the device. By adopting the three-dimensional microfluidic device and method for generating the high-flux micro-droplets, the disadvantage that the speed for generating the micro-droplets by a two-dimensional flow channel at present is slow is overcome, and the generation of high-flux micro-droplets is realized; the whole structure is PDMS and is very firm after the PDMS is mutually bonded, so that a phenomenon that the flow channels are cracked due to excessive pressure of input fluid between the flow channels is avoided; and the PDMS is thoroughly transparent so that the device is easier to observe.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a three-dimensional microfluidic device and method for generating high-throughput microdroplets. Background technique [0002] The droplet microfluidic platform has become an important tool in the fields of biology, chemistry, and medicine. As an important technology in the droplet microfluidic platform, the microdroplet generation method is very important, and different methods differ in the droplet generation rate. very big. At present, the generation methods of micro-droplets mainly include co-flow method, flow convergence method, T-shaped flow channel method and derivative methods based on the above methods. By adjusting the input pressure of gas phase (dispersed phase) and liquid phase (continuous phase), The gas phase can be quickly sheared into microbubbles by the liquid phase, which is usually oil and aqueous solution. For example, 10 11 bubbles (Jeong H H, Chen ...

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Problems solved by technology

However, in the above methods, most of the microfluidic devices used are two-dimensional flow channels, and their generation rate is limited, which is not suitable for the generation of large-flux micro-droplets, especially when large-scale material manufacturing is required. The generation rate is difficult to meet the needs

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