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Application of peripheral blood mononuclear cells hsa-miR-8774-3p as marker of active tuberculosis

A technology for active pulmonary tuberculosis and nuclear cells, applied in the field of biomarkers, can solve the problems of long time-consuming sputum culture, difficult screening, and easy missed detection

Pending Publication Date: 2020-01-03
GUANGDONG MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, sputum smear microscopy and sputum culture are still the most widely used methods for clinical diagnosis of TB, but its shortcomings are also very obvious. The sensitivity of sputum smear microscopy is too low and it is easy to miss detection; sputum culture Time-consuming and difficult to use for TB screening

Method used

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  • Application of peripheral blood mononuclear cells hsa-miR-8774-3p as marker of active tuberculosis
  • Application of peripheral blood mononuclear cells hsa-miR-8774-3p as marker of active tuberculosis
  • Application of peripheral blood mononuclear cells hsa-miR-8774-3p as marker of active tuberculosis

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Embodiment 1

[0031]The application of hsa-miR-874-3p in peripheral blood mononuclear cells as a marker of active pulmonary tuberculosis. The cell line THP-1 was significantly upregulated, and the upregulated hsa-miR-874-3p may act on ATG16L1mRNA in mononuclear macrophages to regulate macrophage autophagy and participate in the infection process of Mtb. The up-regulated hsa-miR-874 has certain significance for the early diagnosis of APTB patients. Therefore, detecting the expression of hsa-miR-874 in APTB patients by qRT-PCR method can be used to diagnose the disease.

Embodiment 2

[0033] The application of hsa-miR-874-3p in peripheral blood mononuclear cells in the preparation of active tuberculosis markers, the applicant first studied hsa-miR-874-3p expression levels in PBMCs of HC and APTB patients 874-3p as a potential biomarker for the early diagnosis of APTB, and found that hsa-miR-874-3p was significantly upregulated in PBMC from APTB patients and the human mononuclear macrophage cell line THP-1 infected with Mycobacterium tuberculosis.

Embodiment 3

[0035] The application of peripheral blood mononuclear cells hsa-miR-874-3p in the preparation of active pulmonary tuberculosis diagnostic reagents, the applicant evaluated the possible interaction between hsa-miR-874-3p and the target ATG16L1 of this miRNA, and proved that hsa -miR-874-3p can participate in the regulation of tuberculosis infection by regulating macrophage autophagy.

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Abstract

The invention relates to the technical field of biomarkers, in particular to application of a peripheral blood mononuclear cell hsa-miR-874-3p as a marker of active tuberculosis. The hsa-miR-874-3p issignificantly up-regulated in peripheral blood mononuclear cells from patients with active tuberculosis and a human monocyte cell line THP-1 infected with tuberculosis, and the up-regulated hsa-miR-874-3p possibly acts on ATG16L1 mRNA in mononuclear macrophages to regulate macrophage autophagy and participate in the infection process of Mtb. The up-regulated hsa-miR-874 has certain significance for the early diagnosis of APTB patients. The detection of the expression condition of hsa-miR-874 in patients with APTB using a qRT-PCR method can diagnose the disease. A new molecular target for clinical diagnosis and treatment is provided, and the development of new drugs is facilitated.

Description

technical field [0001] The invention relates to the technical field of biomarkers, in particular to the application of peripheral blood mononuclear cell hsa-miR-874-3p as a marker of active pulmonary tuberculosis. Background technique [0002] Tuberculosis (TB) remains one of the most pressing public health problems worldwide, causing illness to millions of people every year. China is one of the countries with a high burden of tuberculosis. According to the latest WHO data, in 2016, tuberculosis killed 1.5 million people, including about 890,000 men, 480,000 women and 140,000 children. In recent years, although almost all cases are eventually curable, TB remains one of the greatest threats to humanity, with major public health implications. Future goals should focus on reducing TB incidence, not just new drug development. Early accurate diagnosis and timely treatment are the key to TB control. At present, sputum smear microscopy and sputum culture are still the most wide...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/113
CPCC12Q1/6883C12Q2600/178C12Q2600/158Y02A50/30
Inventor 徐军发罗厚龙罗红张俊爱
Owner GUANGDONG MEDICAL UNIV
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