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A kind of cultivation method for microorganism cultivation

A microbial culture and microorganism technology, applied in biochemical equipment and methods, enzymology/microbiology devices, bioreactors/fermenters for specific purposes, etc., can solve the problem of bacterial death and pollution, high cost, bacterial death and other problems, to achieve good development speed, save culture time, and ensure the effect of culture quality.

Active Publication Date: 2021-09-21
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a method that can avoid the cost caused by the preservation and recovery process of bacteria in view of the high cost caused by the preservation and recovery process of the bacteria in the current microbial culture, and the risk of death and contamination of the bacteria. Microbial culture systems with high risk of strain death and contamination

Method used

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  • A kind of cultivation method for microorganism cultivation
  • A kind of cultivation method for microorganism cultivation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1, such as figure 1 and 2 Shown:

[0080] A cylinder for cultivating microorganisms, comprising a cylinder 1 with a closed lower end and an open upper end and a cover 2 matched with the open end of the upper end of the cylinder 1, and the inner space of the cylinder 1 is divided from bottom to top into Culture substrate chamber 3 and gas chamber 4, described culture substrate chamber 3 is used for containing the culture medium suitable for microbial growth, and described gas chamber 4 is used for containing the suitable gas suitable for microbial growth, also is provided with in described barrel 1 A gas replacement device, the gas replacement device is used to replace the gas in the gas chamber 4 with a new suitable gas.

[0081] In the scheme of this application, the culture medium suitable for microorganism cultivation is the current conventional culture medium, which can be a liquid medium, or a solid medium or a colloidal medium. For different types of mic...

Embodiment 2

[0083] Example 2, such as figure 1 and 2 Shown by:

[0084] A gas replacement device for the culture cylinder of the above embodiment, the gas replacement device includes a replacement component and a gas discharge component, the gas discharge component is used to communicate the gas chamber 4 with the external space of the cylinder body 1, the The replacement component is used to push the gas in the gas cavity 4, so that the gas in the gas cavity 4 is discharged out of the barrel 1 by the gas discharge component.

[0085] As a preferred embodiment, the gas exhaust assembly includes a communication pipe 5, one end of which communicates with the gas chamber 4, and the other end communicates with the external space of the cylinder 1 through the cylinder 1 .

[0086] As a preferred embodiment, a cut-off valve is also provided on the communicating pipe 5 . The communication and blocking states of the communication pipe 5 are controlled by the opening and closing of the cut-off...

Embodiment 3

[0104] Example 3, such as figure 1 and 2 Shown by:

[0105] A microbial culture system, including the above-mentioned microbial culture tube, also includes a temperature control cabin 15, the temperature control cabin 15 has a temperature control chamber 16 capable of maintaining a stable temperature in the cavity, all or the lower part of the culture tube As for the inside of the temperature control chamber 16 . In microbial culture, the temperature of the medium and the surrounding environment of the microorganism is also one of the important factors. Whether the temperature is suitable determines the quality of the growth and development of the microorganism. In actual microbial cultivation, some microorganisms will absorb heat, resulting in the temperature of the culture substrate Some microorganisms will release heat, leading to an increase in the temperature of the culture substrate. These changes in temperature often inhibit the further growth and development of micro...

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Abstract

The present invention relates to the technical field of microorganism cultivation, in particular to a cultivation method for microorganism cultivation. After obtaining microorganism strains, the strains are cultivated in a microorganism cultivation system, and then the microorganism cultivation system cultivated with microorganism strains is transported to On-site use, and then the microorganisms in the culture system are taken out for on-site use. The microbial culture system of the present application directly avoids the steps of preservation and recovery of microorganisms in the traditional way, so that the cost of preservation and recovery is firstly avoided, and more importantly, the death and recovery of microorganisms caused in the traditional preservation and recovery processes are avoided. The risk of variation, and with the method of this application, since the microbial strains are cultivated, they are directly entered into the cultivation system for microbial cultivation, which reduces the risk of microbial variation.

Description

technical field [0001] The invention relates to the technical field of microorganism cultivation, in particular to a cultivation method for microorganism cultivation. Background technique [0002] For microorganisms, it is a large group of organisms including bacteria, viruses, fungi, and some small protozoa, which widely exist in the biological world. Although the individual microorganisms are small, they are closely related to human life and are widely involved. Medicine and health, industry and agriculture, environmental protection and many other fields. [0003] Over the years, microbiologists have carried out scientific experiments in various aspects such as classification, physiology, and genetics, studied the physiological and biochemical characteristics of microorganisms, and summed up a set of microbial detection, screening and cultivation methods, so that microorganisms can be cultivated according to people's needs. People need services. [0004] The current micr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12M1/38C12M1/04C12M1/02C12M1/00C12M1/34
CPCC12M23/58C12M29/06C12M29/14C12M29/20C12M41/22C12M41/40C12M41/46C12N1/20
Inventor 肖英平杨华卢立志吕文涛熊六凤汪雯吴酬飞
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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