Integrative single-cell and cell-free plasma RNA analysis

A cell and collection technology, which is applied in the analysis of materials, biomaterial analysis, character and pattern recognition, etc., can solve the problems that cannot directly evaluate cell aberrations, achieve the effect of reducing noise and simplifying the screening process

Inactive Publication Date: 2020-03-06
THE CHINESE UNIVERSITY OF HONG KONG
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these require assessment by a trained operator and cannot directly assess cellular aberrations

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  • Integrative single-cell and cell-free plasma RNA analysis
  • Integrative single-cell and cell-free plasma RNA analysis
  • Integrative single-cell and cell-free plasma RNA analysis

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[0062] Cells passively or actively release cellular nucleic acid molecules (DNA or RNA) into the extracellular environment. These extracellular free nucleic acid molecules can be detected in circulating plasma. In pregnancy, the estimated fraction of fetal-derived RNA increases from only 3.7% in the first trimester to 11.28% in the third trimester (1, 2). Since RNA transcripts are cell type specific, we thought it would be possible to infer cell type specific changes and aberrations by profiling the profiles of multiple episomal RNA transcripts in plasma specific to the cell type of interest without direct Sample tissue.

[0063] In the setting of pregnancy health assessment, several groups have explored the use of fetal-specific DNA polymorphisms, organ-specific DNA methylation (3), patterns of DNA fragmentation (4, 5), and tissue-specific RNA transcription (2) to isolate the placental contribution in the pool of circulating cell-free fetal nucleic acid and to obtain the ov...

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Abstract

The present invention provides a method of identifying an expressed marker to differentiate between different levels of a condition, comprising analyzing and comparing cell-free RNA molecules reads from different regions of a biological sample.

Description

Background technique [0001] An individual's health depends on the proper functioning and interaction of the different organ systems in the body. Each organ system is composed of multicellular tissues specialized for this purpose. In one estimate, the human body is made up of an average of 37.2 trillion cells. Four basic tissue types have been identified in humans, namely epithelial tissue, connective tissue, nervous tissue and muscle tissue. Human disease arises from abnormalities in cellular function or development. In cancer, vulnerable cells acquire damaging genetic and epigenetic changes in the genome. Such alterations result in altered gene expression and cause abnormal proliferation or other hallmarks of cancer cell behavior. [0002] In one example, one of the main functions of the hematopoietic system is to maintain proper turnover in circulation of blood tissue as a whole, and human blood contains different types of blood cells. Centrifugation can separate human ...

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12Q1/6883C12Q1/6886G01N33/00
CPCC12Q1/6883C12Q1/6886C12Q2600/106C12Q2600/112C12Q2600/118C12Q1/6809G16B20/00G16B25/10C12Q2535/122C12Q2539/107C12Q2563/159G01N33/5091G01N2800/60G01N2800/52G01N2800/56G06V20/698G16B25/00G16B40/00G16B40/20G16B40/30G06F18/2115G01N33/0068
Inventor卢煜明曾卓豪江培勇吉璐王思朗
OwnerTHE CHINESE UNIVERSITY OF HONG KONG