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50 results about "RNA analysis" patented technology
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One of the most common and basic types of RNA analysis is sequence analysis. RNA is made up of four types of molecules known as nucleotides: adenine, guanine, cytosine, and uracil. Determining the sequence of nucleotides in such chains of RNA allows researchers to predict the structure of a resulting protein or to look for mutations in the sequence.
Microfluidic rare cell detection device
The present invention relates to microfluidic devices and methods for detecting rare cells. The disclosed microfluidic devices and methods integrate and automate sample preparation, cell labeling, cell sorting and enrichment, and DNA / RNA analysis of sorted cells.
Owner:PERKINELMER HEALTH SCIENCES INC
Methods for RNA analysis
ActiveUS20160304938A1Easy translationPromote localizationSugar derivativesComponent separationRNA analysisBiology
The present invention relates to the field of RNA analysis. In particular, the invention concerns the use of a catalytic nucleic acid molecule for the analysis of an RNA molecule. The invention concerns methods for analyzing the 5′ terminal structures of an RNA molecule having a cleavage site for a catalytic nucleic acid molecule. In particular, the invention concerns a method for determining the presence of a cap structure in an RNA molecule having a cleavage site for a catalytic nucleic acid molecule, a method for determining the capping degree of a population of RNA molecules having a cleavage site for a catalytic nucleic acid molecule, a method for determining the orientation of the cap structure in a capped RNA molecule having a cleavage site for a catalytic nucleic acid molecule and a method for determining relative amounts of correctly capped RNA molecules and reverse-capped RNA molecules in a population of RNA molecules, wherein the population comprises correctly capped and / or reverse-capped RNA molecules that have a cleavage site for a catalytic nucleic acid molecule. Moreover, the present invention provides uses of a catalytic nucleic acid molecule.
Owner:CUREVAC REAL ESTATE GMBH
Crude biological derivatives competent for nucleic acid detection
InactiveUS20050277121A1Increase concentrationLow number of cycleMicrobiological testing/measurementDNA preparationNucleic acid detectionBiological unit
The invention relates generally to the fields of making biological unit lysates or admixtures of body fluids and of RNA analysis. More specifically, it relates to direct methods for the detection of a specific sequence of RNA in a biological unit, for example a virus, cell or tissue sample, or a body fluid, for example saliva, sputum, blood plasma, etc. More generally, the invention may be used to enzymatically manipulate and protect the RNA in lysate or bodily fluids for a number of applications.
Owner:APPL BIOSYSTEMS INC
Non-invasive prenatal genetic diagnosis using transcervical cells
InactiveUS20050181429A1Microbiological testing/measurementDisease diagnosisPrenatal diagnosisStaining
A non-invasive, risk-free method of prenatal diagnosis is provided. According to the method of the present invention transcervical specimens are subjected to trophoblast-specific immuno-staining followed by FISH, PRINS, Q-FISH and / or MCB analyses and / or other DNA-based genetic analysis in order to determine fetal gender and / or identify chromosomal and / or DNA abnormalities in a fetus. Also provided is a method of in situ chromosomal, DNA and / or RNA analysis of a prestained specimen by incubating the prestained specimen in ammonium hydroxide. Also provided is a method of identifying embryonic cells according to a nucleus / cytoplasm ratio of at least 1:1 and the presence of at least variably condensed chromatin.
Owner:MONALIZA MEDICAL
Compositions and methods of RNA analysis
PendingUS20180208967A1Reduce probabilityIncrease volumeMicrobiological testing/measurementRNA analysisMolecular probe
The present disclosure relates to compositions and methods of RNA analysis. In particular, the present disclosure provides a method of RNA analysis that includes obtaining a sample, applying one or more multi-partite probes to the sample, where each of the one or more multi-partite probes includes at least two sub-probes, annealing at least one of the applied one or more multi-partite probes to at least one target nucleic acid within the sample, and ligating the at least two sub-probes associated with the at least one annealed multi-partite probe to create a target nucleic acid proxy that can be detected.
Owner:THE JOHN HOPKINS UNIV SCHOOL OF MEDICINE
RNA analysis by total hydrolysis
The present invention relates to analysis of an RNA molecule. It further relates to the use of this method for the quality control of an RNA molecule produced by in vitro transcription or for the quality control of an RNA molecule produced by chemical synthesis.
Owner:CUREVAC SE
Sample preparation for in situ nucleic acid analysis
Sample preparation processes for in situ RNA or DNA analysis, methods and compositions therefor are provided. Processes provided herein allow DNA or RNA analysis to be carried out in the same tube or on an aliquot of the prepared sample without centrifugation or extraction. The preparation process can be carried out at room temperature in as little as seven minutes and is amenable to high throughput processing using manual or robotic platforms.
Owner:APPL BIOSYSTEMS INC
cDNA amplification for expression profiling
ActiveUS20040180372A1Consistent outputQuick buildMicrobiological testing/measurementFermentationRNA analysisOligonucleotide
This invention presents a new cDNA amplification method. RNA is first converted to cDNA. The synthesis of cDNA can include a promoter tagged oligonucleotide, and then this cDNA is ligated to form circles (or possibly concatemers). This is then amplified using a Phi29 DNA polymerase based rolling circle and strand displacement amplification. The invention allows for RNA promoter sequences to be attached to the cDNA to facilitate additional amplification through the generation of RNA from the amplified cDNA. The resulting product can then be used to make materials for gene expression studies or other RNA analysis procedures.
Owner:GLOBAL LIFE SCI SOLUTIONS USA LLC
Nucleic acid amplification with integrated multiplex detection
InactiveUS20120094298A1Reduce in quantityEasy to detectMicrobiological testing/measurementGenomic DNAAllelotype Analysis
A method mediated with in-vitro transcription (“IVT”) which permits miniaturization of multiplexed DNA and RNA analysis, and in which elongation-mediated multiplexed analysis of polymorphisms (eMAP®) is used as the analysis step, is described. Also described is a method mediated with IVT is for selecting a designated strand from T7-tagged double stranded DNA: wherein, the selected strand forms the template for RNA synthesis. In one embodiment, double stranded DNA incorporating the T7 (or other) promoter sequence at the 3′ end or the 5′end is produced, for example, by amplification of genomic DNA using the Polymerase Chain Reaction (PCR). Also disclosed are nested PCR designs permitting allele analysis in combination with strand selection by IVT. Further, in one embodiment of a homogeneous format for transcription-mediated amplification and multiplexed detection (which may be particularly suited for viral or pathogen detection), encoded microparticles display “looped” capture probe configurations permitting the generation of a signal upon capture of RNA product and real-time assay monitoring.
Owner:BIOARRAY SOLUTIONS
Non-invasive prenatal genetic diagnosis using transcervical cells
InactiveUS20060040305A1Microbiological testing/measurementDisease diagnosisPrenatal diagnosisCervical cell
A non-invasive, risk-free method of prenatal diagnosis is provided. According to the method of the present invention cell specimens are subjected to molecular and morphological methods which allow trophoblast identification. Trophoblasts identified according to the teachings of the present invention can be further examined to thereby prenatally diagnosing a fetus. Also provided is a method of in situ chromosomal, DNA and / or RNA analysis of a prestained specimen by incubating the prestained specimen in ammonium hydroxide. Also provided is a method of identifying embryonic cells according to a nucleus / cytoplasm ratio of at least 0.3 and the presence of at least variably condensed chromatin.
Owner:MONALIZA MEDICAL
Nucleic acid amplification with integrated multiplex dectection
ActiveUS20060188896A1Reduce riskMicrobiological testing/measurementFermentationGenomic DNAAllelotype Analysis
A method mediated with in-vitro transcription (“IVT”) which permits miniaturization of multiplexed DNA and RNA analysis, and in which elongation-mediated multiplexed analysis of polymorphisms (eMAP®) is used as the analysis step, is described. Also described is a method mediated with IVT is for selecting a designated strand from T7-tagged double stranded DNA: wherein, the selected strand forms the template for RNA synthesis. In one embodiment, double stranded DNA incorporating the T7 (or other) promoter sequence at the 3′ end or the 5′ end is produced, for example, by amplification of genomic DNA using the Polymerase Chain Reaction (PCR). Also disclosed are nested PCR designs permitting allele analysis in combination with strand selection by IVT. Further, in one embodiment of a homogeneous format for transcription-mediated amplification and multiplexed detection (which may be particularly suited for viral or pathogen detection), encoded microparticles display “looped” capture probe configurations permitting the generation of a signal upon capture of RNA product and real-time assay monitoring.
Owner:BIOARRAY SOLUTIONS
Methods for RNA analysis
ActiveUS10648017B2Specific and rapid analysisIncrease contentSugar derivativesComponent separationRNA analysisOrganic chemistry
Owner:CUREVAC REAL ESTATE GMBH
Methods for RNA analysis
InactiveUS20200216878A1Specific and rapid analysisIncrease contentSugar derivativesComponent separationMolecular analysisRNA analysis
The present invention relates to the field of RNA analysis. In particular, the invention concerns the use of a catalytic nucleic acid molecule for the analysis of an RNA molecule. The invention concerns methods for analyzing the 5′ terminal structures of an RNA molecule having a cleavage site for a catalytic nucleic acid molecule. In particular, the invention concerns a method for determining the presence of a cap structure in an RNA molecule having a cleavage site for a catalytic nucleic acid molecule, a method for determining the capping degree of a population of RNA molecules having a cleavage site for a catalytic nucleic acid molecule, a method for determining the orientation of the cap structure in a capped RNA molecule having a cleavage site for a catalytic nucleic acid molecule and a method for determining relative amounts of correctly capped RNA molecules and reverse-capped RNA molecules in a population of RNA molecules, wherein the population comprises correctly capped and / or reverse-capped RNA molecules that have a cleavage site for a catalytic nucleic acid molecule. Moreover, the present invention provides uses of a catalytic nucleic acid molecule.
Owner:CUREVAC REAL ESTATE GMBH
Integrated functional and molecular profiling of cells
PendingCN107667178APolypeptide with localisation/targeting motifImmunoglobulin superfamilyDiseaseMotility
Presented herein are methods of evaluating cellular activity by: placing a cell population on an area; assaying for a dynamic behavior of the cell population as a function of time; identifying cell(s)of interest based on the dynamic behavior; characterizing a molecular profile of the cell(s); and correlating the obtained information. The assayed dynamic behavior can include cellular activation, cellular inhibition, cellular interaction, protein expression, protein secretion, cellular proliferation, changes in cellular morphology, motility, cell death, cell cytotoxicity, cell lysis, and combinations thereof. Sensors associated with the area may be utilized to facilitate assaying. Molecular profiles of the cell(s) can then be characterized by various methods, such as DNA analysis, RNA analysis, and protein analysis. The dynamic behavior and molecular profile can then be correlated for various purposes, such as predicting clinical outcome of a treatment, screening cells, facilitating a treatment, diagnosing a disease, and monitoring cellular activity.
Owner:UNIV HOUSTON SYST
Tivozanib response prediction
A diagnostic method for predicting whether a human tumor will be sensitive or resistant to treatment with tivozanib (AV-951) is disclosed. The method is based on measurement of macrophage content in a tissue sample from a tumor. Measurement of macrophage content can be based on analysis of macrophage marker gene expression, e.g., by RNA analysis or immunohistochemistry.
Owner:AVEO PHARM INC
Amplification of self-ligated, circularized cDNA for expression profiling
ActiveUS7309571B2Reduce concentrationMinimize concatenationMicrobiological testing/measurementFermentationRNA analysisOligonucleotide
This invention presents a new cDNA amplification method. RNA is first converted to cDNA. The synthesis of cDNA can include a promoter tagged oligonucleotide, and then this cDNA is ligated to form circles (or possibly concatemers). This is then amplified using a Phi29 DNA polymerase based rolling circle and strand displacement amplification. The invention allows for RNA promoter sequences to be attached to the cDNA to facilitate additional amplification through the generation of RNA from the amplified cDNA. The resulting product can then be used to make materials for gene expression studies or other RNA analysis procedures.
Owner:GLOBAL LIFE SCI SOLUTIONS USA LLC
Systems and Methods For RNA Analysis In Functional Confirmation Of Cancer Mutations
Owner:FIVE3 GENOMICS
Sample preparation for in situ nucleic acid analysis, methods and compositions therefor
Sample preparation processes for in situ RNA or DNA analysis, methods and compositions therefor are provided. Processes provided herein allow DNA or RNA analysis to be carried out in the same tube or on an aliquot of the prepared sample without centrifugation or extraction. The preparation process can be carried out at room temperature in as little as seven minutes and is amenable to high throughput processing using manual or robotic platforms.
Owner:APPL BIOSYSTEMS INC
Methods for RNA analysis
PendingUS20220073962A1Easy to adaptDirection easyComponent separationMicrobiological testing/measurementChemical MoietyNucleotide
The present invention is concerned with methods for analyzing RNA molecules. The provided methods involve conjugates for RNA cleavage comprising a chemical moiety with RNA cleaving activity and an oligonucleotide. The oligonucleotide is designed based on a target sequence present in an RNA molecule, and the cleavage of the RNA molecule is inter alia carried out at conditions allowing the hybridization of the oligonucleotide to the target 5 sequence. Thereby, the method is easily applicable to RNA molecules of any sequence. The method further involves the analysis of the RNA fragments obtained after cleavage to obtain information on the physical properties of the RNA molecule.
Owner:CUREVAC SE
Tivozanib response prediction
A diagnostic method for predicting whether a human tumor will be sensitive or resistant to treatment with tivozanib (AV-951) is disclosed. The method is based on measurement of macrophage content in a tissue sample from a tumor. Measurement of macrophage content can be based on analysis of macrophage marker gene expression, e.g., by RNA analysis or immunohistochemistry.
Owner:AVEO PHARM INC
Methods and compositions for quantifying immune cell nucleic acids
Provided herein is an RNA analysis method for detecting and quantifying RNA (such as RNA from an immune cell or from a cancer cell) and / or for identifying and quantifying immune cell types from which the RNA originated. In some embodiments, expression levels of genes differentially expressed between healthy subjects and subjects having a disease or disorder are determined based on the RNA. In some embodiments, immune cell types from which the RNA originated are identified and quantified. Provided herein are also methods for determining the likelihood that a subject has the disease or disorder, such as a cancer.
Owner:GUARDANT HEALTH INC
Crude biological derivatives competent for nucleic acid detection
ActiveUS20100167287A1Precise functionMicrobiological testing/measurementFermentationNucleic acid detectionBiological unit
The invention relates generally to the fields of making biological unit lysates or admixtures of body fluids and of RNA analysis. More specifically, it relates to direct methods for the detection of a specific sequence of RNA in a biological unit, for example a virus, cell or tissue sample, or a body fluid, for example saliva, sputum, blood plasma, etc. More generally, the invention may be used to enzymatically manipulate and protect the RNA in lysate or bodily fluids for a number of applications.
Owner:APPL BIOSYSTEMS INC
Integrative single-cell and cell-free plasma RNA analysis
InactiveUS20180372726A1Strong signalSimplify the screening processMicrobiological testing/measurementBiostatisticsLymphatic SpreadCell free
Embodiments of the present technology involve integrative single-cell and cell-free plasma RNA transcriptomics. Embodiments allow for the determination of expressed regions that can be used to identify, determine, or diagnosis a condition or disorder in a subject. Methods described herein analyze cell-free RNA molecules for certain expressed regions. The specific expressed regions analyzed were previously determined to be indicative for a certain type of cell or grouping of cells. As a result, the amounts of cell-free reads at the specific expressed regions may be related to the number of cells in a tissue or organ. The number of cells in the tissue or organ may change as a result of cell death, metastasis, or other dynamics. A change in the number of cells in the tissue or organ may then be reflected in certain expressed regions in cell-free RNA.
Owner:THE CHINESE UNIVERSITY OF HONG KONG
Compositions and methods of RNA analysis
The present invention relates to compositions and methods of RNA analysis. In particular, the present invention provides a method of RNA analysis. The method comprises the steps of: obtaining a sample, applying one or more multi-partite probes to the sample, wherein each of the one or more multi-partite probes includes at least two sub-probes, annealing at least one of the applied one or more multi-partite probes to at least one target nucleic acid within the sample, and ligating the at least two sub-probes associated with the at least one annealed multi-partite probe to create a target nucleic acid proxy that can be detected.
Owner:THE JOHN HOPKINS UNIV SCHOOL OF MEDICINE
Method and system for predicting binding site of non-targeted probing RNA-binding proteins on RNA
ActiveCN120072034BImprove forecast accuracyImprove analytical performanceBiostatisticsMachine learningStructure analysisBinding site
This invention discloses a method and system for predicting RNA binding protein binding sites on RNA without targeting, relating to the field of bioinformatics. The method includes the following steps: acquiring raw RNA structure data; expanding and filtering the preprocessed data; training an RNA analysis model using the filtered data; processing the RNA to be analyzed using the trained RNA analysis model, calculating the probability that each nucleotide is single-stranded, and obtaining a structure score; identifying low-accessibility regions based on the difference between the structure score output by the RNA analysis model and the structure score calculated by the original method; and identifying potential RNA binding protein binding sites by comparing the signal difference with that of the original method in a single RNA structure detection experiment. This invention utilizes the STONE method to improve the accuracy of RNA structure analysis in low-accessibility regions, and uses the improved difference to infer RBP binding sites, thereby improving the accuracy of RBP binding site identification.
Owner:SHANDONG UNIV
Compositions comprising a sequence specific endoribonuclease and methods of use
PCT designated stageWO2025168812A1HydrolasesMicrobiological testing/measurementEndoribonucleaseRibonuclease
The present disclosure provides compositions comprising sequence specific endoribonuclease and methods of their use in RNA analysis, RNA synthesis and fingerprinting of RNA molecules. In particular the present disclosure relates to compositions and samples comprising isolated endoribonuclease of Type III toxin-antitoxin systems preferably endoribonucleases of subfamily CptN and subfamily TenpN.
Owner:ARCTICZYMES
Novel plasma extracellular vesicle long-chain RNA analysis and cancer early diagnosis method
The invention discloses a novel plasma extracellular vesicle long-chain RNA (Ribonucleic Acid) analysis and cancer early diagnosis method, relates to the technical field of cancer biomarkers and classified biology, and is used for carrying out early diagnosis and prediction on PDAC based on plasma EVs long-chain RNA (EvlRNA). According to the method, a two-layer classifier is established, the first layer identifies abnormal and normal samples, and the second layer identifies whether the samples belong to PDAC or CP; according to the invention, a new concept, namely EvlRNA-Index, is provided for the first time, a cancer diagnosis model is established based on the EvlRNA-Index, and a good diagnosis effect is achieved. According to the research, the interpretability of a whole machine learning framework is researched and discussed, close association between important characteristics and an actual biological process is explored, 49 PDAC cancer related genes are identified and obtained, and important help is provided for biomedical researchers to understand a model.
Owner:WANNAN MEDICAL COLLEGE
Compositions Comprising Sequence-Specific Endoribonucleases and Methods of Use
The present disclosure provides compositions comprising sequence-specific endoribonucleases and methods for their use in RNA analysis, RNA synthesis, and fingerprinting of RNA molecules. In particular, the present disclosure relates to compositions and samples comprising ToxN endoribonucleases that recognize and cleave single-stranded RNA, as well as optimal conditions for obtaining cleavage.
Owner:ARCTICZYMES
Multi-omics chip based on spatial interleaved coding and its preparation method and application
The present invention discloses a multi-omics chip based on spatial interleaved coding, its preparation method, and its application. Based on 3D inkjet in situ DNA synthesis technology, the present invention utilizes a spatial interleaved coding strategy to produce a high-resolution, large-scale spatial multi-omics chip. This chip then uses a spatial in situ capture strategy to perform high-throughput in situ transcriptome analysis, in situ epigenomic analysis, in situ non-coding RNA analysis, and in situ proteome analysis on tissue sections. This achieves single-cell resolution and large-scale spatial coverage while maintaining a high gene count detection rate. The chip can be used to construct three-dimensional, fully covered, precise transcriptional maps or spatiotemporal maps of organs or tissues.
Owner:SHANGHAI JIAOTONG UNIV +1