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Applications of five rhodotorula glutinis deacetylated protease homologous genes and products thereof

A homologous gene, Rhodotorula viscosus technology, applied in the field of genetic engineering

Pending Publication Date: 2020-03-17
广州溯原生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To date, no studies have pointed to a link between SIRT4 or SIRT5 genes and anti-aging, but the potential role of SIRT4 and SIRT5 in regulating mitochondrial metabolism is not difficult to associate with the potential impact of these sirtuins in aging-related diseases

Method used

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  • Applications of five rhodotorula glutinis deacetylated protease homologous genes and products thereof
  • Applications of five rhodotorula glutinis deacetylated protease homologous genes and products thereof
  • Applications of five rhodotorula glutinis deacetylated protease homologous genes and products thereof

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Embodiment Construction

[0030] The present invention will be described in further detail below in conjunction with the examples.

[0031] Example Rhodotorula viscosus Sir2 homologous gene influences its own replication lifespan

[0032] 1. Experimental materials

[0033] 1.1 Strain, plasmid

[0034] Table 1 Strains and plasmids

[0035]

[0036] 1.2 Main reagents

[0037] The main reagents in this paper are shown in Table 2:

[0038] Table 2 Experimental Reagents

[0039]

[0040] 1.3 Main instruments

[0041] The main reagents in this paper are shown in Table 3:

[0042] Table 3 Experimental Instruments

[0043]

[0044] 1.4 Main solution and culture medium

[0045] (1) Polyethylene glycol (PEG3350, 50%w / v): 10g polyethylene glycol, diluted to 50% with water, and sterilized by filtration with a 0.45 μm filter membrane. The PEG solution is sealed and stored to prevent moisture from evaporating.

[0046] (2) G418 solution: 10g of G418, prepared with water to a concentration of 100 m...

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Abstract

The invention discloses five rhodotorula glutinis sir2 homologous genes capable of prolonging service life of cells and an application of products of the five rhodotorula glutinis sir2 homologous genes, which belongs to the technical field of biology. According to the invention, five rhodotorula glutinis sir2 homologous genes hst3, hst4, sirtuin 1, sirtuin 2 and sirtuin 4 with prolonged cell lifeare knocked out in rhodotorula glutinis to observe the effect of the rhodotorula glutinis sir2 homologous genes. The experimental results show that knockout of the five genes can shorten the life of yeast cells; bacterial fat accumulation is reduced, ATP generation influence is reduced, key enzyme activity in TCA circulation is reduced, the product has functions of yeast cell life prolonging, ATPgeneration improving and key enzyme activity increasing in TCA circulation, and a new idea is provided for cell aging treatment.

Description

technical field [0001] The invention belongs to the field of genetic engineering. More specifically, the present invention relates to hst3, hst4, sirtuin1, sirtuin 2, sirtuin 4 The application of five kinds of rhodotorula viscosus sir2 homologous genes and their expression products has the functions of prolonging the lifespan of yeast cells, increasing the production of ATP, and increasing the activity of key enzymes in the TCA cycle. Background technique [0002] Evidence that Sirtuins genes in lower eukaryotes are related to lifespan provides a basis for mammalian Sirtuins genes to affect lifespan in mammals. Various experiments have proved that SIRT1 can be used for anti-aging. Recent studies have shown that brain-specific SIRT1 overexpression can prolong lifespan and delay aging in mice. Embryo development and postnatal life are severely impaired in inbred mice lacking SIRT1. A recent study confirmed that outbred mice lacking SIRT1 do not extend their lifespan thro...

Claims

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Application Information

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IPC IPC(8): C12N15/55C12N9/80A61K38/50A61P39/06A61P3/00A23L33/195
CPCC12N9/80A61P39/06A61P3/00A23L33/195A61K38/00A23V2002/00A23V2200/302A23V2200/30
Inventor 孙晗笑刘梦鸽利时雨
Owner 广州溯原生物科技股份有限公司
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