Nereis succinea antioxidant peptide and preparation method and application thereof

An antioxidant peptide and nereid technology, which is applied to the field of nereid antioxidant peptide and its preparation, can solve the problems of staying at the laboratory research level and difficult to produce active substances on a large scale, and achieves good antioxidant effect and low cost. , the preparation method is simple

Active Publication Date: 2020-03-24
山东省海洋科学研究院青岛国家海洋科学研究中心
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, scholars at home and abroad have also carried out related research on the biological characteristics, life history, and large-scale breeding of clamworms. However, the above-mentioned active substances are difficult to produce and prepare on a large scale, and most of them are still at the level of laboratory research.

Method used

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  • Nereis succinea antioxidant peptide and preparation method and application thereof
  • Nereis succinea antioxidant peptide and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0023] A preparation method of Nereis worm antioxidant peptide of the present invention comprises the following steps:

[0024] 1) Nereis, with a body weight of 300-400 tails / Kg, washed after collection, spit sand in seawater at room temperature for 24 hours; take 1Kg clam, add distilled water at a mass ratio of 1:8, and beat for 2 minutes at a speed of 800r / min , adjust the pH value to 2.0 to obtain nereis serous fluid;

[0025] 2) Add pepsin to the clam worm slurry obtained in step 1), the amount of enzyme added is 600U / g feed solution, enzymolysis at 40°C for 4 hours, and inactivation at 90°C for 6 minutes, cooling to obtain enzymolysis liquid;

[0026] 3) The enzymolysis solution obtained in step 2) is roughly filtered with a 200-mesh filter bag to obtain 7.80 L of the crude filtrate;

[0027] 4) The crude filtrate obtained in step 3) is clarified with a ceramic membrane, the parameters of the ceramic membrane are a membrane core precision of 220nm, and a membrane area o...

Embodiment 2

[0063] A preparation method of Nereis worm antioxidant peptide of the present invention comprises the following steps:

[0064] 1) Nereis, with a body mass of 300-400 tails / kg, washed after collection, and spit sand in seawater at room temperature for 48 hours; take 2Kg clam, add distilled water at a mass ratio of 1:6, and beat for 6 minutes at a speed of 1200r / min , adjust the pH value to 3.0 to obtain nereis serous fluid;

[0065] 2) Add pepsin to the clam worm slurry obtained in step 1), the amount of enzyme added is 1000U / g feed solution, enzymolysis at 45°C for 1 hour, and at 100°C for 4 minutes, cooling to obtain enzymolysis liquid;

[0066] 3) The enzymolysis solution obtained in step 2) is roughly filtered with a 100-mesh filter bag to obtain 7.50 L of the crude filtrate;

[0067] 4) The crude filtrate obtained in step 3) is clarified with a ceramic membrane, the parameters of the ceramic membrane are a membrane core precision of 180nm, and a membrane area of ​​0.8m ...

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Abstract

The invention puts forward a Nereis succinea antioxidant peptide and a preparation method and application thereof. The preparation method of the invention comprises the following steps: 1) taking liveNereis succinea, conditioning and pulping to obtain a serous fluid; 2) adding 600-1000 U / g of pepsase into the serous fluid, carrying out enzymolysis at 40-45 DEG C for 1-4 h, carrying out enzyme deactivation at 90-100 DEG C for 4-6 min, and cooling to obtain an enzymatic hydrolysate; and 3) carrying out coarse filtration on enzymatic hydrolysate, and carrying out fine filtration, ultrafiltration, concentration, desalination, purification and drying to obtain the Nereis succinea antioxidant peptide. The preparation method of the invention is simple, is convenient to operate, is green and environment-friendly, and is easy to realize industrialization. The Nereis succinea antioxidant peptide has high purity, strong activity and good antioxidant effect, has scavenging effect on DPPH free radicals, superoxide free radicals and hydroxyl free radicals, and has an obvious protective effect on oxidative damage of HUVEC cells. The Nereis succinea antioxidant peptide can be widely used in the production of nutritional food, functional food and feeds.

Description

technical field [0001] The invention relates to the technical field of polypeptide preparation, in particular to a nereis worm antioxidant peptide and its preparation method and application. Background technique [0002] In taxonomy, clam worms belong to the phylum Annelida, polychaetes, wandering orders, and nereisidae. They are commonly known as sea worms, sea maggots, sea centipedes, and sea leeches, and they inhabit mud and sand. The clamworm is one of the main components of the marine food chain, and it is also an important species for marine ecological restoration and environmental conditions. There are more than 80 species of clam worms in my country, most of which are produced along the coasts of the Yellow Sea and the Bohai Sea. Lugworm is an important economic polychaete. Because of its high export economic value and large export volume, large-scale artificial breeding has been carried out along the coast of Hainan, Fujian, Zhejiang, Guangdong, and Shandong, and i...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K7/06C07K1/36C07K1/34C07K1/24C07K1/16C07K1/14
CPCC12P21/06C07K7/06
Inventor 王颖刘天红李晓刘洪军李红艳
Owner 山东省海洋科学研究院青岛国家海洋科学研究中心
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