Animal cell adherent culture machine

Abstract

The invention discloses an animal cell adherent culture machine. The structure comprises caster wheels, a rack, a driving frame, a culture structure and a control box. Caster wheels are horizontally mounted at the lower end of the rack. The driving frame is horizontally mounted among the rack. The culture structure is located at the upper end of the driving frame. The control box is mounted at thelower end of the rack. The driving frame comprises a mounting frame, a roller, a front arc port and a rear arc port. The roller is mounted among the mounting frame. The front arc port is arranged atthe front end of the mounting frame. The rear arc port is located at the rear end of the mounting frame. The culture structure comprises a culture bottle, a bottle mouth, a communication structure anda breather pipe. The bottle mouth is arranged at the right end of the culture bottle. The communication structure is embedded in the inner side of the bottle mouth. The communication structure is arranged between the bottle mouth and the breather pipe, so that when cells alternately contact with a culture solution and air in the bottle, the cells can directly contact with the outside air to directly obtain oxygen, thus preventing the reduction of inner oxygen and the increase of carbon dioxide, affecting the continuous culture of the animal cells in the bottle, and accelerating the culture process of the animal cells.

Description

technical field [0001] The invention belongs to the field of cell culture, and in particular relates to an animal cell adherent culture machine. Background technique [0002] Cell culture is mainly to simulate the internal environment in the equipment to carry out the structure of survival, growth, reproduction and maintenance, and there are many kinds of cells, so the environment produced in the cell culture is also different, and it includes the attachment of cells Wall culture, so that cells attach to the inside of the container with culture medium, undergo mitosis and enter the logarithmic growth phase, and in the adherent culture of cells, it usually requires alternating contact between culture medium and air, so animal cell stickers are required Wall culture machine, but there are following deficiencies in the prior art: [0003] Because animal cells in adherent culture usually absorb oxygen in the air and produce carbon dioxide while absorbing substances in the nutri...

AI Technical Summary

Problems solved by technology

[0003] Since animal cells in adherent culture usually absorb the substances in the nutrient solution, they will also absorb oxygen in the air and produce carbon dioxide, and the cells are usually in contact with the culture solution and the air in the bottle alternately, so the bottle rotates. If it is airtight, the oxygen in the bottle will continue to decrease and the carbon dioxide will also increase, resulting in insufficient oxygen in the bottle and the need to open the bottle again, which will affect the process of animal cell culture

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