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Method for rapidly breeding hybrid orchid by root, as well as culture medium

A technology of hybrid orchid and culture medium, applied in the field of plant tissue culture, can solve the problems of never using roots as explants, difficulty in initial culture, complicated culture process, etc., so as to expand the source of explants and the survival rate of transplanting. 100%, the effect of speeding up the training process

Inactive Publication Date: 2015-04-15
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The explants are mainly stem tips and lateral buds, which cause great damage to the plants; the explants are prone to browning after inoculation, and the initial cultivation is difficult; the cultivation process is complicated and the reproduction coefficient is low; it is the rapid propagation of hybrid orchid tissue culture at present. main problem
[0006] Using roots as explants for tissue culture has the unique advantages of large amount of material, easy acquisition, and little damage to plants. However, there are no reports or invention patents of using roots as explants in the current research on tissue culture of hybrid orchids.

Method used

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  • Method for rapidly breeding hybrid orchid by root, as well as culture medium
  • Method for rapidly breeding hybrid orchid by root, as well as culture medium
  • Method for rapidly breeding hybrid orchid by root, as well as culture medium

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Use the root of the hybrid orchid test-tube seedling as material, cut it into sections and inoculate it, culture it in the dark at a temperature of about 25°C (25±2°C), and induce protocorms. The protocorm induction medium is MS (Murashige and Skoog, 1962)+6 -BA (6-benzylaminoadenine) 0.5mg / L+PIC (4-amino-3,5,6-trichloropyridine-2-acid) 0.6mg / L+hydrolyzed casein 200mg / L+sucrose 30000mg / L+agar Powder 5000mg / L. After 12 days, the root segment expanded and began to produce protocorms, such as figure 1 As shown, protocorms proliferated in large quantities and gradually differentiated into shoots, as shown in figure 2 Shown, protocorm induction rate 100%.

[0036] Transfer the protocorm induction medium that has induced and multiplied a large number of protocorms and sprouts to the light at a temperature of about 25°C (25±2°C). After 3 days, the protocorms and sprouts begin to turn green and gradually develop into clusters seedlings, while protocorms continue to prolifer...

Embodiment 2

[0040] Take the root of the test-tube plantlet obtained in Example 1 as material, inoculate after cutting into sections, culture in dark at a temperature of about 25°C (25±2°C), induce protocorms, and the protocorm induction medium is MS+6-BA0.5mg / L+PIC0.6mg / L+hydrolyzed casein 200mg / L+sucrose 30000mg / L+agar powder 5000mg / L, protocorm induction rate is 100%. After 30 days of culture, select the differentiated sprouts and move them to the new protocorm induction medium MS+6-BA0.5mg / L+PIC0.6mg / L+hydrolyzed casein 200mg / L+sucrose 30000mg / L+agar powder 5000mg / L Light culture, while leaving the undifferentiated protocorms on the original medium to continue to proliferate, differentiate and then transfer to light culture, and then adopt the same culture steps as in Example 1. After about 80 days of culture, each root explant has an average More than 100 plants can be induced to root.

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Abstract

The invention discloses a method for rapidly breeding a hybrid orchid by a root, as well as a culture medium, and belongs to the technical field of plant tissue culture. The method comprises steps as follows: a root segment of the hybrid orchid is selected and inoculated on an induction culture medium for dark culture, and a protocorm is induced; after a large amount of the protocorms are proliferated, the protocorms are transferred for light culture and develop into tufted seedlings; and the tufted seedlings are cut into single seedlings and inoculated to a strong seedling rooting culture medium, and a rooting plant is cultured and obtained. According to the method, MS (murashige and skoog) is taken as a basic culture medium, 6-BA (6-benzyladenine), PIC (4- amino-3,5,6-trichloropyridine-2-acid) and casein hydrolysate are required to be added to the induction culture medium, and the strong seedling rooting culture medium is the MS culture medium without hormone. According to the method and the culture medium, the breeding efficiency is high, the operation is simple and convenient, and all of the protocorm inductivity, the plant rooting rate and the transplanting survival rate of root explants can reach 100%.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method and culture medium for rapid propagation of hybrid orchids by using roots. Background technique [0002] Hybrid orchid is a new type of orchid selected from the hybridization of Chinese orchid and Cymbidium [Lu Jiliang. A preliminary study on the production and marketing status of Yunnan hybrid orchid [J]. Huamu Bonjing (Flower Gardening), 2013, (2): 36-37; Liang Fang , Cui Bo, Ma Jie, Ye Yongzhong. Proliferation and differentiation of hybrid orchid protocorms [J]. Anhui Agricultural Sciences, 2008, 36(13): 5309-5310, 5353]. Hybrid orchids are medium-sized, colorful, fragrant and elegant, making up for the lack of fragrance of Cymbidium. High ornamental value and broad market prospects [Zhang Xianyun, Yuan Xiuyun, Ma Jie. Research on tissue culture and rapid propagation technology of hybrid orchids [J]. Henan Science, 2009, 27(4): 419-421; Lu Ra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 张建军周音陈敏敏谢纪红
Owner SHANGHAI ACAD OF AGRI SCI
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